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A Biocompatible Condensation Reaction for the Labeling of Terminal Cysteine Residues on Proteins

2009; Wiley; Volume: 48; Issue: 51 Linguagem: Inglês

10.1002/anie.200903627

1521-3773

Hongjun Ren, Fei Xiao, Ke Zhan, Young‐Pil Kim, Hexin Xie, Zuyong Xia, Jianghong Rao,

Monoclonal and Polyclonal Antibodies Research

Going live: A protein-labeling method based on the use of a single amino acid tag—an N-terminal cysteine residue—and small-molecule probes containing a cyanobenzothiazole (CBT) unit has been used for the specific fluorescence labeling of proteins in vitro and at the surface of live cells (see scheme). This simple ligation reaction proceeds with a high degree of specificity under physiological conditions. Rd: a rhodamine dye; TEV: tobacco etch virus.