Decrease in apparent K m for oxygen after stimulation of respiration of rat polymorphonuclear leukocytes
1983; Wiley; Volume: 161; Issue: 1 Linguagem: Inglês
10.1016/0014-5793(83)80730-3
ISSN1873-3468
AutoresSteven W. Edwards, Maurice B. Hallett, David Lloyd, Anthony K. Campbell,
Tópico(s)Immune Response and Inflammation
ResumoThe respiratory burst of polymorphonuclear leukocytes, induced by the addition of chemotactic peptide (N-formyl-methionyl-leucyl-phenylalanine) and cytochalasin B was found to consist of two phases. The first phase of very rapid oxygen uptake lasted 1-3 min. and was followed by a second more prolonged phase of lower magnitude. The apparent Km for oxygen of unstimulated cells was 9.6 +/- 0.67 microM, while that of the second phase of stimulation was 3.7 +/- 1.6 microM oxygen. The possibility that lowered oxygen concentrations may regulate polymorphonuclear leukocyte activity in some pathological conditions is discussed.
Referência(s)