Sensitive and selective determination of picogram amounts of ciprofloxacin and its metabolites in biological samples using high-performance liquid chromatography and photothermal post-column derivatization
1987; Elsevier BV; Volume: 416; Linguagem: Inglês
10.1016/0378-4347(87)80515-7
ISSN1872-812X
AutoresH. Scholl, Kathrin Schmidt, B. Weber,
Tópico(s)Analytical Chemistry and Chromatography
ResumoAn analytical method for the detection and quantification of ciprofloxacin and its known metabolites M1, M2, M3 and M4 in urine, serum/plasma, bile, faeces and tissue is described. For the liquid matrices the only sample preparation that is required is dilution. The assay consists of reversed-phase high-performance liquid chromatography and fluorescence detection. For the metabolites M2, M3 and M4 an additional post-column derivatization by successive thermolysis and photolysis is needed. A suitable simple post-column reactor has been constructed. Detection limits for all compounds are between 0.2 and 2.2 ng/ml (absolute detection limits 2–22 pg). Compared with fluorescence detection alone, the derivatization increases the selectivity of detection significantly. The linearity, precision and accuracy of the method were determined.
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