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Evidence for a quinone binding site close to the interface between NUOD and NUOB subunits of Complex I

2001; Elsevier BV; Volume: 1504; Issue: 2-3 Linguagem: Inglês

10.1016/s0005-2728(01)00158-x

1879-2650

Isabelle Prieur, Joël Lunardi, Alain Dupuis,

Mitochondrial Function and Pathology

Piericidin, rotenone and pyridaben are specific inhibitors of the NADH–ubiquinone oxidoreductase (Complex I) that bind to its ubiquinone binding site(s). Using site directed mutagenesis, we demonstrate that residues G409, D412, R413 and V407 of the C-terminus of Complex I NUOD subunit are directly involved in the binding of these inhibitors. We propose that the corresponding inhibitor/quinone binding site would be located close to NUOD–NUOB interface.