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Generation of Multinucleated Giant Cells In Vitro from Bovine Monocytes and Macrophages

2004; Japanese Society of Veterinary Science; Volume: 66; Issue: 9 Linguagem: Inglês

10.1292/jvms.66.1065

1347-7439

Kazuhiro Yoshihara, Reiko Nagata, Yoshihiro Muneta, Shigeki Inumaru, Yuichi Yokomizo, Yasuyuki Mori,

Immunotherapy and Immune Responses

The generation of multinucleated giant cells (MGC) from cells of the bovine monocyte-macrophage lineage was investigated. Freshly isolated monocytes were incubated with the conditioned medium (CM) of peripheral blood mononuclear cell cultures treated with Concanavalin A for 1-4 days (CM1 to CM4). Only CM1 generated MGC despite similar concentrations of IFNγ in all CMs. Nevertheless, MGC formation from monocytes was enhanced by adding either macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF), MGC formations from macrophages were observed only when macrophages were cultured with GM-CSF plus CM. These results indicate that several mechanisms to generate MGC from bovine monocytes-macrophage lineage cells exist, and that GM-CSF is a major mediator of MGC formation in cattle.