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Transgenic Mouse Technology in Skin Biology: Generation of Knockin Mice

2014; Elsevier BV; Volume: 134; Issue: 12 Linguagem: Inglês

10.1038/jid.2014.434

1523-1747

Frederik Tellkamp, Farida Benhadou, Jeroen Bremer, Maria Gnarra, Jana Knüver, Sandra Schaffenrath, Susanne Vorhagen,

CRISPR and Genetic Engineering

Genetic modification of model organisms is a powerful tool to study human diseases in vivo. Mice have become the main subject of interest for basic researchers because they share a broad spectrum of physiological and pathological similarities with humans. These similarities, genetic accessibility, and a defined genetic background within the laboratory mouse strains provide the basis to analyze in vivo cellular and molecular pathways that are altered in human diseases. Knockout and knockin mice have become valuable tools to investigate the role of certain proteins and thereby have helped researchers to overcome cause and consequence problems arising from biochemical data (Hacking, 2008).