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Metabolomic profiling of asthma: Diagnostic utility of urine nuclear magnetic resonance spectroscopy

2011; Elsevier BV; Volume: 127; Issue: 3 Linguagem: Inglês

10.1016/j.jaci.2010.12.1077

1097-6825

Erik J. Saude, Christopher D. Skappak, Shana Regush, Kim K. Cook, Amos Ben‐Zvi, Allan B. Becker, Redwan Moqbel, Brian D. Sykes, Brian H. Rowe, Darryl J. Adamko,

Chronic Obstructive Pulmonary Disease (COPD) Research

Background The ability to diagnose and monitor asthma on the basis of noninvasive measurements of airway cellular dysfunction is difficult in the typical clinical setting. Objective Metabolomics is the study of molecules created by cellular metabolic pathways. We hypothesized that the metabolic activity of children with asthma would differ from healthy children without asthma. Furthermore, children having an asthma exacerbation would be different compared with children with stable asthma in outpatient clinics. Finally, we hypothesized that 1 H-nuclear magnetic resonance (NMR) would measure such differences using urine samples, one of the least invasive forms of biofluid sampling. Methods Children (135 total, ages 4-16 years) were enrolled, having met the criteria of healthy controls (C), stable asthma in the outpatient clinic (AO), or unstable asthma in the emergency department (AED). Partial least squares discriminant analysis was performed on the NMR data to create models of separation (70 metabolites were measured/urine sample). Some NMR data were withheld from modeling to be run blindly to determine possible diagnostic accuracy. Results On the basis of the model of AO versus C, 31 of 33 AO samples were correctly diagnosed with asthma (94% accuracy). Only 1 of 20 C samples was incorrectly labeled as asthma (5% misclassification). On the basis of the AO versus AED model, 31 of the 33 AO samples were correctly diagnosed as outpatient asthma (94% accurate). Conclusion This is the first report suggesting that 1 H-NMR analysis of human urine samples has the potential to be a useful clinical tool for physicians treating asthma.