Characterization of a monoclonal antibody recognizing a polymorphic epitope mainly on HLA‐DPw2 and DPw4 molecules
1989; Wiley; Volume: 34; Issue: 4 Linguagem: Inglês
10.1111/j.1399-0039.1989.tb02100.x
ISSN1399-0039
AutoresHelge D. Viken, Gustav Gaudernack, Erik Thorsby,
Tópico(s)T-cell and B-cell Immunology
ResumoA monoclonal antibody (MAb), 13.3.B4, was obtained from a murine hybridoma after fusing Sp2/0 myeloma ceUs with spleen cells from C3H mice immunized with mouse L cells transfected with the A1 and B1 genes of HLA‐DPw4. In radiobinding‐assays, MAb 13.3.B4 bound to HLA transfectants expressing DPw2 or DPw4 as well as DPw2 or DPw4 homozygous B‐cell lines, while most cell lines expressing other DP determinants were negative. MAbs with known DP or other HLA class II‐specificities were used to inhibit binding of MAb 13.3.B4 in a radioimmunoassay. Three MAbs demonstrated inhibition, but their pattern of reactivity with HLA homozygous B cell lines differed from that of MAb 13.3.M. An evaluation of DNA sequence data showed that MAb 13.3.B4 reacts with all cell lines expressing DP β‐chains of type 2.1, 2.2.4.1 or 4.2. No correlation between 13.3.B4‐reactivity and expression of DP α‐chain variants was found. The results indicate that MAb 13.3.B4 defines a polymorphic epitope which may be determined by the sequence gly‐gly‐pro‐met at residues 84‐87 of the DP β‐chain.
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