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Glutathione content of cultured human fibroblasts during in vitro ageing

1984; Elsevier BV; Volume: 27; Issue: 3 Linguagem: Inglês

10.1016/0047-6374(84)90055-1

1872-6216

Martin Poot, Anton Verkerk, Johan F. Jongkind,

Immunotherapy and Immune Responses

The glutathione level of cultured human fibroblasts was determined with a micro-modification of a spectrophotometric glutathione cycling method. There was a slight increase in reduced glutathione (GSH) content during in vitro ageing of normal human fibroblasts. Fibroblasts from patients with Werner's syndrome of ceroid lipofuscinosis (Spielmeyer-Vogt syndrome) and healthy individuals exhibited similar patterns of GSH levels during in vitro ageing. The GSH content of non-proliferating confluent cultures of normal fibroblasts and of proliferating normal fibroblasts was identical. Moreover, autofluorescent “aged” cells isolated by cell sorting did not differ in GSH content from the non-autofluorescent cells in the same culture. It was concluded that the GSH content does not play a role in in vitro ageing, nor in the accumulation of autofluorescent material in human skin fibroblasts.