Artigo Revisado por pares

Sucrose interference in the assay of enzymes and protein

1969; Elsevier BV; Volume: 29; Issue: 2 Linguagem: Inglês

10.1016/0003-2697(69)90308-x

ISSN

1096-0309

Autores

Richard H. Hinton, Malcolm Leonard Ernest. Burge, GORDON C. HARTMAN,

Tópico(s)

Metabolomics and Mass Spectrometry Studies

Resumo

Sucrose has been found to interfere with the estimation of protein and with the assay of a variety of soluble and particulate enzymes. No interference was detected in the estimation of inorganic phosphate by the Lowry and Lopez procedure. The usual pattern of interference was a reversible reduction of activity, linearly related to sucrose concentration.

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