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Biomedical applications of high-performance liquid chromatography—mass spectrometry with continuous-flow fast atom bombardment

1991; Elsevier BV; Volume: 562; Issue: 1-2 Linguagem: Inglês

10.1016/0378-4347(91)80563-r

1872-812X

David S. Millington, Daniel L. Norwood, Naoki Kodo, Raymond C. Moore, Michael D. Green, Judd Berman,

Analytical Chemistry and Chromatography

This report describes the application of high-performance liquid chromatography combined with continuous-flow fast atom bombardment mass spectrometry to analytical problems in the biomedical laboratory. Applications include the compound-specific detection of diagnostic acylcarnitines in human urine, the separation and analysis of acyl-coenzyme A thioesters, and qualitative studies on complex mixtures of modified peptides (dansyl and dinitrophenyl derivatives). For each of these applications standard analytical columns (3.9 mm I.D.) and 1 ml/min flow-rates were employed with post-column stream splitting (1:100) before mass spectrometry. Various mobile phase compositions and solvent gradients were employed. The addition of 1-5% glycerol to the mobile phase was shown to have little effect on the chromatography. For all compounds studied (acylcarnitines, acyl-coenzyme A thioesters, and derivatized peptides) molecular weight information was obtained and sufficient sensitivity was achieved to allow unambiguous identification of trace components in complex mixtures.