Portal de Periódicos da CAPES

Comparison of Hydrogels in the In Vivo Formation of Tissue-Engineered Bone Using Mesenchymal Stem Cells and Beta-Tricalcium Phosphate

2007; Mary Ann Liebert, Inc.; Volume: 13; Issue: 4 Linguagem: Inglês

10.1089/ten.2006.0083

1557-8690

Christian Weinand, Rajiv Gupta, Albert Y. Huang, Eli J. Weinberg, Ijad Madisch, Rameez A. Qudsi, Craig M. Neville, Irina Pomerantseva, Joseph P. Vacanti,

Periodontal Regeneration and Treatments

Availability of grafts and morbidity at the donor site limit autologous transplantation in patients requiring bone reconstruction. A tissue-engineering approach can overcome these limitations by producing bone-like tissue of custom shape and size from isolated cells. Several hydrogels facilitate osteogenesis on porous scaffolds; however, the relative suitability of various hydrogels has not been rigorously assessed. Fibrin glue, alginate, and collagen I hydrogels were mixed with swine bone marrow–derived differentiated mesenchymal stem cells (MSCs), applied to 3-dimensionally printed porous beta-tricalcium phosphate (β-TCP) scaffolds and implanted subcutaneously in nude mice. Although noninvasive assessment of osteogenesis in 3 dimensions is desirable for monitoring new bone formation in vivo, correlations with traditional histological and mechanical testing need to be established. High-resolution volumetric computed tomography (VCT) scanning, histological examination, biomechanical compression testing, and osteonectin (ON) expression were performed on excised scaffolds after 1, 2, 4, and 6 weeks of subcutaneous implantation in mice. Statistical correlation analyses were performed between radiological density, stiffness, and ON expression. Use of collagen I as a hydrogel carrier produced superior bone formation at 6 weeks, as demonstrated using VCT scanning with densities similar to native bone and the highest compression values. Continued contribution of the seeded MSCs was demonstrated using swine-specific messenger ribonucleic acid probes. Radiological density values correlated closely with the results of histological and biomechanical testing and ON expression. High-resolution VCT is a promising method for monitoring osteogenesis.