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Evaluation of hepatoprotective effect of Gentiana olivieri herbs on subacute administration and isolation of active principle

2003; Elsevier BV; Volume: 72; Issue: 20 Linguagem: Inglês

10.1016/s0024-3205(03)00117-6

1879-0631

Didem Deliorman Orhan, Mustafa Aslan, Göknur Aktay, Ender Ergun, Erdem Yeşilada, Fatma Ergün,

Medicinal plant effects and applications

Hepatoprotective effect of Gentiana olivieri Griseb. (Gentianaceae) flowering herbs on subacute administration were studied using in vivo models in rats. For the activity assessment on carbon tetrachloride-induced hepatic damage following biochemical parameters were evaluated; plasma and hepatic tissue malondialdehyde formation, and liver tissue glutathione level, as well as plasma transaminase enzyme levels (aspartate transferase and alanine transferase). Results of biochemical tests were also confirmed by histopathological examination. Through bioassay-guided fractionation procedures isoorientin, a known C-glycosylflavone, was isolated from the ethyl acetate fraction as the active antihepatotoxic constituent by silica gel column chromatography. Isoorientin exhibited significant hepatoprotective effect at 15 mg/kg b.w. dose.