Transcriptomic correlates of organ failure extent in sepsis
2014; Elsevier BV; Volume: 70; Issue: 5 Linguagem: Inglês
10.1016/j.jinf.2014.12.010
ISSN1532-2742
AutoresRaquel Almansa, María Heredia‐Rodríguez, Esther Gómez‐Sánchez, David Andaluz‐Ojeda, Verónica Iglesias, Lucía Rico, Alicia Ortega, Estefanía Gómez‐Pesquera, Pilar Liu, M. Fernandez Aragon, José María Eirós Bouza, María A. Jiménez‐Sousa, Salvador Resino, Ignacio Gómez-Herreras, Jesús F. Bermejo-Martín, Eduardo Tamayo,
Tópico(s)Inflammation biomarkers and pathways
ResumoObjectives Sepsis is characterised by the frequent presence of organ failure and marked immunologic alterations. We studied the association between the extent of organ failure and the transcriptomic response of septic patients. Methods Gene expression profiles in the blood of 74 surgical patients with sepsis were compared with those of 30 surgical patients with no sepsis. Differentially expressed genes were assessed for their correlation with the sequential organ failure (SOFA) score. Results The expression levels of a group of genes participating in the cell cycle (HIST1H1C, CKS2, CCNA2, CDK1, CCNB2, CIT, CCNB1, AURKA, RAD51), neutrophil protease activity (ELANE, ADORA3, MPO, MMP8, CTSG), IL-1R and IL-18R response correlated directly with SOFA and mortality. Genes involved in T cell (LCK, CD3G, CD3D, ZAP70, ICOS, CD3E, CD28, IL2RB, CD8B, CD8A, CD40LG, IL23A, CCL5, SH2D1A, ITK, CD247, TBX21, GATA3, CCR7, LEF1, STAT4) and NK cell immunity (CD244, KLRK1, KLRD1) were inversely associated with SOFA and mortality. Conclusions The extent of organ failure in sepsis correlates directly with the existence of imbalanced innate and adaptive responses at the transcriptomic level. Quantification of the expression levels of the genes identified here could contribute to the simultaneous assessment of disease severity and immunological alterations in sepsis.
Referência(s)