Produção Nacional
Revisado por pares
Isoflavone regulates Vascular Endothelial Growth Factor Expression in urinary tract of castrated rats
2009; Elsevier BV; Volume: 62; Issue: 3 Linguagem: Inglês
10.1016/j.maturitas.2009.01.003
ISSN1873-4111
AutoresMaria Dione Dutra Sampaio, Zsuzsanna Ilona Katalin de Jármy Di Bella, Ismael Dale Cotrim Guerreiro da Silva, Elaine Travaglia Santos, Naiara Correa Nogueira-de-Souza, Eliana Viana Monteiro Zucchi, Manuel de Jesus Simões, Manoel Joâo Batista Castello Girão, Marair Grácio Ferreira Sartori,
Tópico(s)Phytoestrogen effects and research
ResumoObjective The purpose of this study was to investigate Vascular Endothelial Growth Factor Expression (VEGF) gene regulation by isoflavone in urinary tract tissues of castrated adult rats. Design Forty-five adult rats, 90 days old, weighting 200 g were used, receiving a soy-free ration. The animals were castrated for drug administration for 30 days (125 μg genisteine/g body weight/day) and sacrificed, divided into three groups: Group I—control; Group II—started isoflavone administration on the 5th day after castration; Group III—started isoflavone administration on the 28th day after castration. RNA was isolated from each bladder and urethra. Determination of VEGF gene regulated by isoflavone was obtained using a semiquantitative RT-PCR and immunohistochemistry of total RNA isolated from bladder and urethra. Results Our results demonstrate that isoflavone was able to upregulate mRNA level of the VEGF gene in the lower urinary tract of rats in Group II, where isoflavone administration was started at an early phase of estrogen deprivation, while in Group III, where isoflavone administration was started in the late phase of hypoestrogenism, did not show alteration of bladder and urethra VEGF gene expression, compared to placebo, maintaining the same level of the castrated rats without treatment. Conclusions The data indicate that VEGF expression in rats is also regulated by isoflavone in early phase of hypoestrogenism.
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