Advances in 2D gel techniques
2000; Elsevier BV; Volume: 18; Linguagem: Inglês
10.1016/s0167-7799(00)00011-1
ISSN0167-9430
Autores Tópico(s)Metabolomics and Mass Spectrometry Studies
ResumoRecent advances have been made in analytical and micropreparative two-dimensional electrophoresis of proteins with immobilized pH gradients, using wide-range gradients up to pH 12 and/or extended separation distances. These advances have helped us to gain an overview of the state of the proteome. In addition, immobilized pH gradients for zoom-in gels offer the optimum resolution and detection of minor components, as well as automated procedures.
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