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Fluorescent Polymeric Transducer for the Rapid, Simple, and Specific Detection of Nucleic Acids at the Zeptomole Level

2004; American Chemical Society; Volume: 126; Issue: 13 Linguagem: Inglês

10.1021/ja038900d

1943-2984

Kim Doré, Sébastien Dubus, Hoang‐Anh Ho, Isabelle Lévesque, Maryse Brunette, Geneviève Corbeil, Maurice Boissinot, Guy Boivin, Michel G. Bergeron, Denis Boudreau, Mario Leclerc,

Biosensors and Analytical Detection

We report the specific detection of a few hundred molecules of genetic material using a fluorescent polythiophene biosensor. Such recognition is based on simple electrostatic interactions between a cationic polymeric optical transducer and the negatively charged nucleic acid target and can be done in less than 1 h, simply and affordably, and without any chemical reaction. This simple system is versatile enough to detect nucleic acids of various lengths, including a segment from the RNA genome of the Influenza virus.