Response surface optimization of ultrasound-assisted enzymatic extraction polysaccharides from Lycium barbarum
2014; Elsevier BV; Volume: 110; Linguagem: Inglês
10.1016/j.carbpol.2014.03.040
ISSN1879-1344
AutoresYong Liu, Guili Gong, Jiao Zhang, Shao‐Yi Jia, Feng Li, Yanyan Wang, Song‐Hai Wu,
Tópico(s)Microbial Metabolites in Food Biotechnology
ResumoIn this study, an efficient ultrasound-assisted enzymatic extraction procedure for the water-soluble polysaccharides from the fruit of Lycium barbarum was investigated and optimized. Response surface methodology (RSM) based on a three-level four-factor Box Behnken Design (BBD) was employed to optimize the extraction conditions inlcluding extraction time, ultrasonic output power, cellulose concentration and extraction temperature. The experimental data were adequately fitted into a second-order polynomial model. The optimized conditions were as follows: extraction time 20.29 min, ultrasonic output power 78.6 W, cellulose concentration 2.15%, extraction temperature 55.79 °C. Under these conditions, the experimental yield of polysaccharides was 6.31 ± 0.03%, which matched with the predictive yield of 6.32% well.
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