Portal de Periódicos da CAPES

Enzymatic determination of cardiac glycosides

1971; Academic Press; Volume: 5; Issue: 4 Linguagem: Inglês

10.1016/0006-2944(71)90031-7

1557-7996

Fedor Medziharadsky, P Nandhasri, Urmilla Khanna,

Protein Hydrolysis and Bioactive Peptides

A specific and highly sensitive enzymatic method for the determination of cardiac glycosides has been developed. The method is based on the inhibition of the Na,K-ATPase in a microsomal preparation from beef cerebral cortex. The procedure, which involves simple laboratory techniques and equipment, has been applied to biological material. One to 20 ng of digoxin or digitoxin were analyzed in human plasma with a standard deviation of ±7.5% to ±2%.