Sample self-stacking in capillary zone electrophoresis: Behavior of samples containing multiple major coionic components*
2000; Wiley; Volume: 21; Issue: 14 Linguagem: Inglês
10.1002/1522-2683(20000801)21
ISSN1522-2683
AutoresPetr Gebauer, Ludmila Křivánková, Pavla Pantůčková, Petr Boček, Wolfgang Thormann,
Tópico(s)Spectroscopy Techniques in Biomedical and Chemical Research
ResumoELECTROPHORESISVolume 21, Issue 14 p. 2797-2808 Research Article Sample self-stacking in capillary zone electrophoresis: Behavior of samples containing multiple major coionic components* Petr Gebauer, Corresponding Author Petr Gebauer [email protected] Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech RepublicInstitute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveří 97, CZ-61142 Brno, Czech Republic Fax: +4205-41212113===Search for more papers by this authorLudmila Křivánková, Ludmila Křivánková Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech RepublicSearch for more papers by this authorPavla Pantůčková, Pavla Pantůčková Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech RepublicSearch for more papers by this authorPetr Boček, Petr Boček Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech RepublicSearch for more papers by this authorWolfgang Thormann, Wolfgang Thormann Department of Clinical Pharmacology, University of Bern, Bern, SwitzerlandSearch for more papers by this author Petr Gebauer, Corresponding Author Petr Gebauer [email protected] Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech RepublicInstitute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveří 97, CZ-61142 Brno, Czech Republic Fax: +4205-41212113===Search for more papers by this authorLudmila Křivánková, Ludmila Křivánková Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech RepublicSearch for more papers by this authorPavla Pantůčková, Pavla Pantůčková Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech RepublicSearch for more papers by this authorPetr Boček, Petr Boček Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech RepublicSearch for more papers by this authorWolfgang Thormann, Wolfgang Thormann Department of Clinical Pharmacology, University of Bern, Bern, SwitzerlandSearch for more papers by this author First published: 24 August 2000 https://doi.org/10.1002/1522-2683(20000801)21:14 3.0.CO;2-PCitations: 59AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract It is a frequent phenomenon in practice that a sample contains bulk levels of more than one coionic component that affect the stacking behavior of minor analytes and in this way also the sensitivity of the method. Here, attention is paid to stacking resulting from the presence of a macrocomponent of leading type that is deteriorated by the presence of another macrocomponent of like charge in the sample. Based on the isotachophoretic model of migration in the initial period of separation, a theoretical approach was elaborated both for strong and weak electrolytes which describes the separation process and finds the conditions that define whether transient isotachophoretic stacking of the analyte takes place or not. It is shown that the crucial parameter is the ratio of the concentrations of macrocomponents migrating in front and behind the analyte of interest. The destacking effect can also be expected when the coion of the background electrolyte is present in the sample. Rules how to cope with effects of destackers present in the sample are given. Theoretical considerations are illustrated by computer simulations and verified experimentally. Examples of antagonistic effects of macrocomponents are demonstrated for model serum samples. Citing Literature Volume21, Issue141 August 2000Pages 2797-2808 RelatedInformation
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