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Sequential Triplex Real-Time PCR Assay for Detecting 21 Pneumococcal Capsular Serotypes That Account for a High Global Disease Burden

2012; American Society for Microbiology; Volume: 51; Issue: 2 Linguagem: Inglês

10.1128/jcm.02927-12

1098-660X

Fabiana Cristina Pimenta, Alexis Roundtree, Ahmet Soysal, Mustafa Bakır, Mignon du Plessis, Nicole Wolter, Anne von Gottberg, Lesley McGee, Maria da Glória Carvalho, Bernard Beall,

Antimicrobial Resistance in Staphylococcus

We developed and validated a real-time PCR assay consisting of 7 triplexed reactions to identify 11 individual serotypes plus 10 small serogroups representing the majority of disease-causing isolates of Streptococcus pneumoniae. This assay targets the 13 serotypes included within the 13-valent conjugate vaccine and 8 additional key serotypes or serogroups. Advantages over other serotyping assays are described. The assay will be expanded to 40 serotypes/serogroups. We will provide periodic updates at our protocol website.