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PROSTATE CANCER DETECTION ON URINALYSIS FOR α METHYLACYL COENZYME A RACEMASE PROTEIN

2004; Lippincott Williams & Wilkins; Volume: 172; Issue: 4 Part 1 Linguagem: Inglês

10.1097/01.ju.0000137659.53129.14

1527-3792

Craig Rogers, Gai Yan, Shan Zha, Mark L. Gonzalgo, William B. Isaacs, Jun Luo, Angelo M. De Marzo, William G. Nelson, Christian P. Pavlovich,

Molecular Biology Techniques and Applications

No AccessJournal of UrologyInvestigative Urology1 Oct 2004PROSTATE CANCER DETECTION ON URINALYSIS FOR α METHYLACYL COENZYME A RACEMASE PROTEIN CRAIG G. ROGERS, GAI YAN, SHAN ZHA, MARK L. GONZALGO, WILLIAM B. ISAACS, JUN LUO, ANGELO M. DE MARZO, WILLIAM G. NELSON, and CHRISTIAN P. PAVLOVICH CRAIG G. ROGERSCRAIG G. ROGERS , GAI YANGAI YAN , SHAN ZHASHAN ZHA , MARK L. GONZALGOMARK L. GONZALGO , WILLIAM B. ISAACSWILLIAM B. ISAACS , JUN LUOJUN LUO , ANGELO M. DE MARZOANGELO M. DE MARZO , WILLIAM G. NELSONWILLIAM G. NELSON , and CHRISTIAN P. PAVLOVICHCHRISTIAN P. PAVLOVICH View All Author Informationhttps://doi.org/10.1097/01.ju.0000137659.53129.14AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: We assessed the feasibility of a novel urinary test for prostate cancer based on the presence of α methylacyl coenzyme A racemase (AMACR) protein in voided urine specimens obtained after prostate biopsy. Materials and Methods: Clean catch voided urine specimens were prospectively collected from 26 consecutive men immediately after transrectal ultrasound guided prostate biopsy for suspected malignancy. The presence of AMACR was evaluated in a blinded manner by Western blot analysis and correlated with biopsy results and patient clinical information. Results: AMACR was detected in the urine in 18 of 26 patients (69%). AMACR was detected in all 12 patients with biopsy confirmed adenocarcinoma of the prostate (100% sensitivity, 95% CI 75 to 100), in 5 of 12 with no evidence of cancer on biopsy (58% specificity, 95% CI 29 to 78) and in 1 of 2 (50%, 95% CI 3 to 80) with atypia on biopsy. Overall AMACR detection was associated with cancer status by prostate biopsy in 21 of 26 patients (86%). Conclusions: We report the feasibility of a novel, noninvasive, nonprostate specific antigen based molecular approach to detect prostate cancer in voided urine. To our knowledge this is the first report of AMACR protein detection in the urine of patients with prostate cancer. A screening test based on urinary AMACR may develop into a useful adjunct to serum prostate specific antigen and digital rectal examination for identifying men at increased risk for harboring prostate cancer despite negative biopsy. Such a test has potential application for stratifying patients into low and high risk groups for surveillance vs repeat biopsy. References 1 : Cancer statistics, 2003. CA Cancer J Clin2003; 53: 5. Google Scholar 2 : Comparison of percent free PSA, PSA density, and age-specific PSA cutoffs for prostate cancer detection and staging. Urology2000; 56: 255. Google Scholar 3 : Serum free prostate specific antigen and prostate specific antigen density measurements for predicting cancer in men with prior negative prostatic biopsies. J Urol1997; 158: 2162. 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Google Scholar 10 : Alpha-methylacyl-CoA racemase: a novel tumor marker over-expressed in several human cancers and their precursor lesions. Am J Surg Pathol2002; 26: 926. Google Scholar 11 : Alpha-methylacyl-CoA racemase: a variably sensitive immunohistochemical marker for the diagnosis of small prostate cancer foci on needle biopsy. Am J Surg Pathol2003; 27: 1128. Google Scholar 12 : Identification of differentially expressed genes in human prostate cancer using subtraction and microarray. Cancer Res2000; 60: 1677. Google Scholar 13 : Subcellular localization and physiological role of α-methylacyl-CoA racemase. J Lipid Res2000; 41: 1890. Google Scholar 14 : Fat, meat, and prostate cancer. Epidemiol Rev2001; 23: 72. Google Scholar 15 : Prostate cancer detection by GST1 methylation analysis of postbiopsy urine specimens. Clin Cancer Res2003; 9: 2673. Google Scholar 16 : Expression of alpha-methylacyl-CoA racemase (P504S) in atypical adenomatous hyperplasia of the prostate. Am J Surg Pathol2002; 26: 921. Google Scholar From the Brady Urological Institute (CGR, GY, SZ, MLG, WBI, JL, AMD, WGN, CPP) and Department of Pathology (ADM, WGN), Johns Hopkins University School of Medicine and Sidney Kimmel Comprehensive Cancer Center (WBI, AMD, WGN), Baltimore, Maryland© 2004 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byOuyang B, Bracken B, Burke B, Chung E, Liang J and Ho S (2018) A Duplex Quantitative Polymerase Chain Reaction Assay Based on Quantification of α-Methylacyl-CoA Racemase Transcripts and Prostate Cancer Antigen 3 in Urine Sediments Improved Diagnostic Accuracy for Prostate CancerJournal of Urology, VOL. 181, NO. 6, (2508-2514), Online publication date: 1-Jun-2009. Volume 172Issue 4 Part 1October 2004Page: 1501-1503 Advertisement Copyright & Permissions© 2004 by American Urological Association, Inc.Keywordsprostatic neoplasmstumor markersprostateurinalysisbiologicalMetricsAuthor Information CRAIG G. ROGERS More articles by this author GAI YAN More articles by this author SHAN ZHA More articles by this author MARK L. GONZALGO More articles by this author WILLIAM B. ISAACS More articles by this author JUN LUO More articles by this author ANGELO M. DE MARZO More articles by this author WILLIAM G. NELSON More articles by this author CHRISTIAN P. PAVLOVICH More articles by this author Expand All Advertisement PDF downloadLoading ...