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EFFECT OF INTENSITY AND WAVELENGTH OF FLUORESCENT LIGHT ON CHROMOSOME DAMAGE IN CULTURED MOUSE CELLS

1979; Wiley; Volume: 29; Issue: 5 Linguagem: Inglês

10.1111/j.1751-1097.1979.tb07800.x

1751-1097

R. Parshad, Katherine K. Sanford, William G. Taylor, Robert E. Tarone, Gary M. Jones, Anne E. Baeck,

Air Quality and Health Impacts

Abstract—A single 3‐ to 20‐hr exposure of line NCTC 9266 mouse cells to cool‐white fluorescent light (4.6 W/m 2 ) produces chromatid breaks and exchanges. The effective wavelength is in the visible range and coincides with the mercury emission peak at 405 nm. Increasing light intensity from 4.6 W to 15.3 W/m 2 for 20 h causes a concomitant increase both in production of chromosome damage and formation of hydrogen peroxide (H 2 O 2 ) in the serum‐free medium. Cells washed free of medium and illuminated in saline for 3 h show chromosome damage to the same extent as cells illuminated in culture medium. Addition of catalase during the exposure period of 3 h eliminates the light‐induced damage. We conclude that the light‐induced chromatid breaks and exchanges result from H 2 O 2 production within the cell and that exogenous catalase can enter the cell and prevent the damage.