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Specification of SUMO1- and SUMO2-interacting Motifs

2006; Elsevier BV; Volume: 281; Issue: 23 Linguagem: Inglês

10.1074/jbc.m512757200

1083-351X

Christina-Maria Hecker, Matthias Rabiller, Kaisa Haglund, Peter Bayer, Ivan Đikić,

Mitochondrial Function and Pathology

SUMO proteins are ubiquitin-related modifiers implicated in the regulation of gene transcription, cell cycle, DNA repair, and protein localization. The molecular mechanisms by which the sumoylation of target proteins regulates diverse cellular functions remain poorly understood. Here we report isolation and characterization of SUMO1- and SUMO2-binding motifs. Using yeast two-hybrid system, bioinformatics, and NMR spectroscopy we define a common SUMO-interacting motif (SIM) and map its binding surfaces on SUMO1 and SUMO2. This motif forms a beta-strand that could bind in parallel or antiparallel orientation to the beta2-strand of SUMO due to the environment of the hydrophobic core. A negative charge imposed by a stretch of neighboring acidic amino acids and/or phosphorylated serine residues determines its specificity in binding to distinct SUMO paralogues and can modulate the spatial orientation of SUMO-SIM interactions.