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Comparison of the functional potencies of ropinirole and other dopamine receptor agonists at human D 2(long) , D 3 and D 4.4 receptors expressed in Chinese hamster ovary cells

1999; Wiley; Volume: 127; Issue: 7 Linguagem: Inglês

10.1038/sj.bjp.0702673

1476-5381

Martyn C. Coldwell, Izzy Boyfield, Tony Brown, Jim J. Hagan, Derek N. Middlemiss,

Adenosine and Purinergic Signaling

The aim of the present study was to characterize functional responses to ropinirole, its major metabolites in man (SKF‐104557 (4‐[2‐(propylamino)ethyl]‐2‐(3H) indolone), SKF‐97930 (4‐carboxy‐2‐(3H) indolone)) and other dopamine receptor agonists at human dopamine D 2(long) (hD 2 ), D 3 (hD 3 ) and D 4.4 (hD 4 ) receptors separately expressed in Chinese hamster ovary cells using microphysiometry. All the receptor agonists tested (ropinirole, SKF‐104557, SKF‐97930, bromocriptine, lisuride, pergolide, pramipexole, talipexole, dopamine) increased extracellular acidification rate in Chinese hamster ovary clones expressing the human D 2 , D 3 or D 4 receptor. The pEC 50 s of ropinirole at hD 2 , hD 3 and hD 4 receptors were 7.4, 8.4 and 6.8, respectively. Ropinirole is therefore at least 10 fold selective for the human dopamine D 3 receptor over the other D 2 receptor family members. At the hD 2 and hD 3 dopamine receptors all the compounds tested were full agonists as compared to quinpirole. Talipexole and the ropinirole metabolite, SKF‐104557, were partial agonists at the hD 4 receptor. Bromocriptine and lisuride had a slow onset of agonist action which precluded determination of EC 50 s. The rank order of agonist potencies was dissimilar to the rank order of radioligand binding affinities at each of the dopamine receptor subtypes. Functional selectivities of the dopamine receptor agonists, as measured in the microphysiometer, were less than radioligand binding selectivities. The results show that ropinirole is a full agonist at human D 2 , D 3 and D 4 dopamine receptors. SKF‐104557 the major human metabolite of ropinirole, had similar radioligand binding affinities to, but lower functional potencies than, the parent compound. British Journal of Pharmacology (1999) 127 , 1696–1702; doi: 10.1038/sj.bjp.0702673