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Distinct transcriptional profiles characterize bone microenvironment mesenchymal cells rather than osteoblasts in relationship with multiple myeloma bone disease

2009; Elsevier BV; Volume: 38; Issue: 2 Linguagem: Inglês

10.1016/j.exphem.2009.11.009

1873-2399

Katia Todoerti, Gina Lisignoli, Paola Storti, Luca Agnelli, Francesca Novara, Cristina Manferdini, Katia Codeluppi, Simona Colla, Monica Crugnola, Manuela Abeltino, Marina Bolzoni, Valentina Sgobba, Andrea Facchini, Giorgio Lambertenghi‐Deliliers, Orsetta Zuffardi, Vittorio Rizzoli, Antonino Neri, Nicola Giuliani,

Bone health and treatments

Objective Multiple myeloma (MM) is characterized by a high incidence of osteolytic bone lesions, which have been previously correlated with the gene expression profiles of MM cells. The aim of this study was to investigate the transcriptional patterns of cells in the bone microenvironment and their relationships with the presence of osteolysis in MM patients. Materials and Methods Both mesenchymal (MSC) and osteoblastic (OB) cells were isolated directly from bone biopsies of MM patients and controls to perform gene expression profiling by microarrays and real-time polymerase chain reaction on selected bone-related genes. Results We identified a series of upregulated and downregulated genes that were differentially expressed in the MSC cells of osteolytic and nonosteolytic patients. Comparison of the osteolytic and nonosteolytic samples also showed that the MSC cells and OB had distinct transcriptional patterns. No significantly modulated genes were found in the OBs of the osteolytic and nonosteolytic patients. Conclusions Our data suggest that the gene expression profiles of cells of the bone microenvironment are different in MM patients and controls, and that MSC cells, but not OBs, have a distinct transcriptional pattern associated with the occurrence of bone lesions in MM patients. These data support the idea that alterations in MSC cells may be involved in MM bone disease.