Antigenic determinant and interspecies cross‐reactivity of a monoclonal antibody to poly(ADP‐ribose) synthetase
1985; Wiley; Volume: 182; Issue: 2 Linguagem: Inglês
10.1016/0014-5793(85)80340-9
ISSN1873-3468
AutoresIsamu Kameshita, Hiroshi Yamamoto, Shigeyoshi Fujimoto, Yutaka Shizuta,
Tópico(s)RNA Interference and Gene Delivery
ResumoA monoclonal antibody (1F4) was prepared against calf thymus poly(ADP‐ribose) synthetase. It was classified as IgG 1 /κ and its antigenic determinant was localized on the 46 kDa portion of the enzyme molecule which contains the site for the binding of DNA. When calf thymus DNA‐binding proteins were subjected to immunostaining after electrophoresis and transblotting to a nitrocellulose filter, the native enzyme (120 kDa) and its endogenous degradation products (80, 64 and 32 kDa) were detected. When the interspecies cross‐reactivity was examined using DNA‐binding proteins from 6 different sources, 1F4 reacted with the 120‐ and 32‐kDa protein bands in HeLa cells, mouse testis and chicken liver as in the case of calf thymus. These results indicate that the antigenic structures ofpoly(ADP‐ribose) synthetase and its degradation products are highly conserved in various animal cells.
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