Biochemical effects of lysergic acid diethylamide on the liver fluke Fasciola hepatica
1970; Elsevier BV; Volume: 19; Linguagem: Inglês
10.1016/0006-2952(70)90374-6
ISSN1873-2968
AutoresTag E. Mansour, Deborah B. Stone,
Tópico(s)Nematode management and characterization studies
ResumoSerotonin (5-hydroxytryptamine) and lysergic acid diethylamide cause an increase in glycolysis in the liver fluke Fasciola hepatica. The effect of serotonin on glycosis appears to be due to an increase in phosphofructokinase activity and is mediated through cyclic 3′,5′-AMP. The biochemical effects of lysergic acid diethylamide on the liver flukes were examined in the present paper. Like serotonin, lysergic acid diethylamide causes an increase in the activity of phosphofructokinase in the liver flukes. This effect can be obtained either by preincubating the intact flukes with lysergic acid diethylamide or by adding it directly to fluke homogenates. While serotonin activates phosphofructokinase through its effect on a particulate fraction from the flukes, lysergic acid diethylamide did not have an effect on this fraction. The question whether lysergic acid diethylamide effect is mediated through serotonin was investigated. The concentration of serotonin in intact liver flukes was found to be 231 ng/g wet weight. The level of serotonin in the flukes is markedly increased when the flukes are incubated with 5-hydroxytryptophan but is unchanged following incubation with tryptophan. Lysergic acid diethylamide does not change the levels of endogenous serotonin in control flukes. It also does not affect the levels of serotonin synthesized by the organism from 5-hydroxytryptophan. The data does not support the idea that the effect of lysergic acid diethylamide is mediated through an increase in serotonin levels although the effects on carbohydrate metabolism of both agents are similar.
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