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Differential Expression of Rat Brain Phospholipase C Isozymes in Development and Aging

1998; Elsevier BV; Volume: 243; Issue: 1 Linguagem: Inglês

10.1006/bbrc.1998.8090

1090-2104

Shun Shimohama, Yasuo Sumida, Sadaki Fujimoto, Yasuji Matsuoka, Takashi Taniguchi, Tadafumi Takenawa, Jun Kimura,

Mitochondrial Function and Pathology

Phosphoinositide-specific phospholipase C (PLC) is a key enzyme in signal transduction. In the present study we examined developmental and aging changes in three PLC isozymes (β1, γ1, and δ1) in the rat brain. Enzyme assays and immunoblot analyses after gel filtration chromatography of brain extracts from embryonic day 19 and postnatal 4- and 48-week rats indicated that γ1-specific activity was highest in fetal brain and decreased with aging, that β1-specific activity was high at 4 weeks but essentially undetected in fetal brain, and that δ1-specific activity was high at both 4 and 48 weeks with faint detection in fetal brain. Our results suggest that the γ1 isozyme may be particularly involved in cell division and growth during the histogenesis of the central nervous system, while β1 and δ1 isozymes may take part in processes of its maturation and maintenance.