Retrospective Application of Transposon-Directed Insertion Site Sequencing to a Library of Signature-Tagged Mini-Tn 5 Km2 Mutants of Escherichia coli O157:H7 Screened in Cattle
2011; American Society for Microbiology; Volume: 193; Issue: 7 Linguagem: Inglês
10.1128/jb.01292-10
ISSN1098-5530
AutoresSabine Eckert, Francis Dziva, Roy R. Chaudhuri, Gemma C. Langridge, Daniel J. Turner, Derek Pickard, Duncan J. Maskell, Nicholas R. Thomson, Mark P. Stevens,
Tópico(s)Enterobacteriaceae and Cronobacter Research
ResumoABSTRACT Massively parallel sequencing of transposon-flanking regions assigned the genotype and fitness score to 91% of Escherichia coli O157:H7 mutants previously screened in cattle by signature-tagged mutagenesis (STM). The method obviates the limitations of STM and markedly extended the functional annotation of the prototype E. coli O157:H7 genome without further animal use.
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