Produção Nacional
Revisado por pares
Anti-inflammatory activity and possible mechanism of extract from Mikania laevigata in carrageenan-induced peritonitis
2009; Oxford University Press; Volume: 61; Issue: 8 Linguagem: Inglês
10.1211/jpp.61.08.0014
ISSN2042-7158
AutoresClaudiney F. Alves, Vanessa Beatriz Freitas Alves, Izabel P. de Assis, Juliana Trindade Clemente‐Napimoga, Elizabeth Uber Bucek, Daniela Dal-Secco, Fernando Q. Cunha, Vera Lúcia Garcia Rehder, Marcelo Henrique Napimoga,
Tópico(s)Bioactive Compounds and Antitumor Agents
ResumoAbstract Objectives The aim was to test the potential use of an extract of Mikania laevigata (popularly known in Brazil as guaco), made from leaves harvested in different months of the year, on neutrophil migration after an inflammatory stimulus and investigate the underlying molecular mechanisms. Methods We examined the effect of guaco on vascular permeability and leucocyte function in carrageenan-induced peritonitis in mice. Key findings Our results demonstrated that guaco extract administered subcutaneously (3 mg/kg) decreased the vascular permeability and also leucocyte rolling and adhesion to the inflamed tissues by a mechanism dependent on nitric oxide. Specifically, inhibitors of nitric oxide synthase remarkably abrogated the guaco extract-mediated suppression of neutrophil migration to the inflammatory site. In addition, guaco extract-mediated suppression of neutrophil migration appeared to be dependent on the production of the cytokines interleukin-1β and tumour necrosis factor-α. One of the major constituents of the guaco extract, coumarin, was able to inhibit the neutrophil migration towards the inflammatory focus. Conclusions In conclusion the anti-inflammatory effect induced by guaco extract may be by inhibition of pro-inflammatory cytokine production at the inflammatory site.
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