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Diversity of endophytic bacteria in medicinally important Nepenthes species

2013; Medknow; Volume: 4; Issue: 2 Linguagem: Inglês

10.4103/0976-9668.117022

2229-7707

Subhash Janardhan Bhore, Vijayan Komathi, KodiI Kandasamy,

Planarian Biology and Electrostimulation

Nepenthes species are used in traditional medicines to treat various health ailments. However, we do not know which types of endophytic bacteria (EB) are associated with Nepenthes spp.The objective of this study was to isolate and to identify EB associated with Nepenthes spp.Surface-sterilized leaf and stem tissues from nine Nepenthes spp. collected from Peninsular Malaysia were used to isolate EB. Isolates were identified using the polymerase chain reaction-amplified 16S ribosomal DNA (rDNA) sequence similarity based method.Cultivable, 96 isolates were analyzed; and the 16S rDNA sequences analysis suggest that diverse bacterial species are associated with Nepenthes spp. Majority (55.2%) of the isolates were from Bacillus genus, and Bacillus cereus was the most dominant (14.6%) among isolates.Nepenthes spp. do harbor a wide array of cultivable endophytic bacteria.