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A portable silicon photodiode luminometer

1987; Wiley; Volume: 1; Issue: 3 Linguagem: Inglês

10.1002/bio.1170010305

1099-1271

Kim Marks, Paul R. Killeen, J. Goundry, John Gibbons, Roger Bunce,

Calibration and Measurement Techniques

Abstract A simple, inexpensive, battery‐powered, portable luminometer which is based on a silicon photodiode is described. The instrument is intended to measure the light produced by chemiluminescent and bioluminescent reactions. The devic shows a good detection limit and, in a bioluminescent reaction for adenosine 5′‐triphosphate (ATP), detected 0.5 pmol in 1ml of aqueous solution. The instrument measures irradiance from 10 −13 to 10− 11 W cm −2 at the sensor, within the range 300 to 900nm.