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Simple and rapid detection of HLA-A*31:01 for prediction of carbamazepine-induced hypersensitivity using loop-mediated isothermal amplification method

2013; Elsevier BV; Volume: 74; Issue: 1 Linguagem: Inglês

10.1016/j.jdermsci.2013.11.013

1873-569X

Hiroyuki Niihara, Kunie Kohno, Takeshi Taketani, Sakae Kaneko, Takafumi Ito, Takashi Sugamori, Nobuyuki Takahashi, Tuyoshi Miyaoka, Shihoh Okazaki, Hideaki YASUDA, Motohide Furuya, Michiharu Nagahama, Eishin Morita,

Allergic Rhinitis and Sensitization

Carbamazepine (CBZ), which is widely used in management of epilepsy or neuropathic pain, causes fatal severe cutaneous adverse reactions (SCARs). CBZ-induced SCARs are known to occur in strong association with human leukocyte antigen (HLA)-A*31:01 in Japanese and European populations. HLA genotyping is currently used to detect human HLA-A*31:01.To establish a simple and rapid screening assay specific for HLA-A*31:01, the loop-mediated isothermal amplification (LAMP) method was employed on a sample Japanese population.A set of LAMP primers targeting exon 2 of HLA-A*31:01 were designed. Thirty-two clinical samples including the representative HLA-A allele in Japan were used to assess the specificity of LAMP primers in the detection of HLA-A*31:01.The HLA-A*31:01-specific LAMP assay showed consistency with polymerase chain reaction reverse sequence-specific oligonucleotide probe (PCR-rSSO) and polymerase chain reaction-sequence based typing (PCR-SBT) results.High sensitivity and specificity of the HLA-A*31:01 LAMP assay was confirmed. Considering its convenience, the assay can be widely used to screen patients at high genetic risk of CBZ-induced SCARs.