A Single PLP‐Dependent Enzyme PctV Catalyzes the Transformation of 3‐Dehydroshikimate into 3‐Aminobenzoate in the Biosynthesis of Pactamycin
2013; Wiley; Volume: 14; Issue: 10 Linguagem: Inglês
10.1002/cbic.201300153
ISSN1439-7633
AutoresAkane Hirayama, Tadashi Eguchi, Fumitaka Kudo,
Tópico(s)Enzyme Structure and Function
ResumoChemBioChemVolume 14, Issue 10 p. 1198-1203 Communication A Single PLP-Dependent Enzyme PctV Catalyzes the Transformation of 3-Dehydroshikimate into 3-Aminobenzoate in the Biosynthesis of Pactamycin Akane Hirayama, Akane Hirayama Department of Chemistry and Materials Science, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551 (Japan)Search for more papers by this authorProf. Dr. Tadashi Eguchi, Corresponding Author Prof. Dr. Tadashi Eguchi [email protected] Department of Chemistry and Materials Science, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551 (Japan) Tadashi Eguchi, Department of Chemistry and Materials Science, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551 (Japan)=== Fumitaka Kudo, Department of Chemistry, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551 (Japan)===Search for more papers by this authorProf. Dr. Fumitaka Kudo, Corresponding Author Prof. Dr. Fumitaka Kudo [email protected] Department of Chemistry, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551 (Japan) Tadashi Eguchi, Department of Chemistry and Materials Science, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551 (Japan)=== Fumitaka Kudo, Department of Chemistry, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551 (Japan)===Search for more papers by this author Akane Hirayama, Akane Hirayama Department of Chemistry and Materials Science, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551 (Japan)Search for more papers by this authorProf. Dr. Tadashi Eguchi, Corresponding Author Prof. Dr. Tadashi Eguchi [email protected] Department of Chemistry and Materials Science, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551 (Japan) Tadashi Eguchi, Department of Chemistry and Materials Science, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551 (Japan)=== Fumitaka Kudo, Department of Chemistry, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551 (Japan)===Search for more papers by this authorProf. Dr. Fumitaka Kudo, Corresponding Author Prof. Dr. Fumitaka Kudo [email protected] Department of Chemistry, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551 (Japan) Tadashi Eguchi, Department of Chemistry and Materials Science, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551 (Japan)=== Fumitaka Kudo, Department of Chemistry, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8551 (Japan)===Search for more papers by this author First published: 06 June 2013 https://doi.org/10.1002/cbic.201300153Citations: 20Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Graphical Abstract Natural amino donation: A PLP-dependent aminotransferase PctV, encoded in the pactamycin biosynthetic gene cluster, was found to catalyze the formation of 3-aminobenzoate from 3-dehydroshikimate with L-glutamate as the amino donor. The PctV reaction comprises a transamination and two dehydration reactions. This is the first report of a simple 3-ABA synthase in nature. Supporting Information As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors. Filename Description cbic_201300153_sm_miscellaneous_information.pdf2.1 MB miscellaneous_information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. References 1C. T. Walsh, S. W. Haynes, B. D. Ames, Nat. Prod. Rep. 2012, 29, 37–59. 2 2aJ. C. Eads, M. Beeby, G. Scapin, T.-W. Yu, H. G. Floss, Biochemistry 1999, 38, 9840–9849; 2bC.-G. Kim, T.-W. Yu, C. B. Fryhle, S. Handa, H. G. Floss, J. Biol. Chem. 1998, 273, 6030–6040. 3H. Suzuki, Y. Ohnishi, Y. Furusho, S. Sakuda, S. Horinouchi, J. Biol. Chem. 2006, 281, 36944–36951. 4 4aB. K. Bhuyan, A. Dietz, C. G. Smith, Antimicrob. Agents Chemother. 1962, 1961, 184–190; 4bP. F. Wiley, H. K. Jahnke, F. MacKellar, R. B. Kelly, A. D. Argoudelis, J. Org. Chem. 1970, 35, 1420–1425. 5 5aD. D. Weller, K. L. Rinehart, Jr., J. Am. Chem. 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