Hormone Binding by Protein Disulfide Isomerase, a High Capacity Hormone Reservoir of the Endoplasmic Reticulum
2001; Elsevier BV; Volume: 276; Issue: 1 Linguagem: Inglês
10.1074/jbc.m007670200
ISSN1083-351X
Autores Tópico(s)Transgenic Plants and Applications
ResumoProtein disulfide isomerase (PDI) is a folding assistant of the eukaryotic endoplasmic reticulum, but it also binds the hormones, estradiol, and 3,3′,5-triiodo-l-thyronine (T3). Hormone binding could be at discrete hormone binding sites, or it could be a nonphysiological consequence of binding site(s) that are involved in the interaction PDI with its peptide and protein substrates. Equilibrium dialysis, fluorescent hydrophobic probe binding (4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid (bis-ANS)), competition binding, and enzyme activity assays reveal that the hormone binding sites are distinct from the peptide/protein binding sites. PDI has one estradiol binding site with modest affinity (2.1 ± 0.5 μm). There are two binding sites with comparable affinity for T3 (4.3 ± 1.4 μm). One of these overlaps the estradiol site, whereas the other binds the hydrophobic probe, bis-ANS. Neither estradiol nor T3 inhibit the catalytic or chaperone activity of PDI. Although the affinity of PDI for the hormones estradiol and T3 is modest, the high local concentration of PDI in the endoplasmic reticulum (>200 μm) would drive hormone binding and result in the association of a substantial fraction (>90%) of the hormones in the cell with PDI. High capacity, low affinity hormone sites may function to buffer hormone concentration in the cell and allow tight, specific binding to the true receptor while preserving a reasonable number of hormone molecules in the very small volume of the cellular environment. Protein disulfide isomerase (PDI) is a folding assistant of the eukaryotic endoplasmic reticulum, but it also binds the hormones, estradiol, and 3,3′,5-triiodo-l-thyronine (T3). Hormone binding could be at discrete hormone binding sites, or it could be a nonphysiological consequence of binding site(s) that are involved in the interaction PDI with its peptide and protein substrates. Equilibrium dialysis, fluorescent hydrophobic probe binding (4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid (bis-ANS)), competition binding, and enzyme activity assays reveal that the hormone binding sites are distinct from the peptide/protein binding sites. PDI has one estradiol binding site with modest affinity (2.1 ± 0.5 μm). There are two binding sites with comparable affinity for T3 (4.3 ± 1.4 μm). One of these overlaps the estradiol site, whereas the other binds the hydrophobic probe, bis-ANS. Neither estradiol nor T3 inhibit the catalytic or chaperone activity of PDI. Although the affinity of PDI for the hormones estradiol and T3 is modest, the high local concentration of PDI in the endoplasmic reticulum (>200 μm) would drive hormone binding and result in the association of a substantial fraction (>90%) of the hormones in the cell with PDI. High capacity, low affinity hormone sites may function to buffer hormone concentration in the cell and allow tight, specific binding to the true receptor while preserving a reasonable number of hormone molecules in the very small volume of the cellular environment. protein disulfide isomerase 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid 3,3′,5-triiodo-l-thyronine endoplasmic reticulum dithiothreitol 17β-estradiol horse liver alcohol dehydrogenase insulin chain B Protein disulfide isomerase (PDI)1 is a versatile and multi-functional folding assistant of the endoplasmic reticulum (1Freedman R.B. Hirst T.R. Tuite M.F. Trends Biochem. Sci. 1994; 19: 331-336Abstract Full Text PDF PubMed Scopus (656) Google Scholar, 2Noiva R. Protein Expression Purif. 1994; 5: 1-13Crossref PubMed Scopus (58) Google Scholar, 3Gilbert H.F. Pain R. Protein Folding. IRL Press Ltd., Oxford1994: 104-136Google Scholar, 4Gilbert H.F. J. Biol. Chem. 1997; 272: 29399-29402Abstract Full Text Full Text PDF PubMed Scopus (241) Google Scholar, 5Gilbert H.F. Guzman N.A. Prolyl Hydroxylase. Marcel Dekker, New York1997: 341-368Google Scholar, 6Walker K.W. Gilbert H.F. Fink A. Goto H. Structure and Function of Molecular Chaperones: The Role of Chaperones in the Life Cycle of Proteins. Marcel Dekker, New York1997: 331-360Google Scholar). Its essential function in eukaryotes (7Laboissiere M.C.A. Sturley S.L. Raines R.T. J. Biol. Chem. 1995; 270: 28006-28009Abstract Full Text Full Text PDF PubMed Scopus (185) Google Scholar) is as a catalyst of disulfide rearrangements (isomerase activity) during oxidative protein folding (ER). There are two remarkable facts about PDI. It is a very abundant protein in the cell; its concentration in the ER lumen has been estimated to be 100–200 μm (8Lyles M.M. Gilbert H.F. Biochemistry. 1991; 30: 613-619Crossref PubMed Scopus (349) Google Scholar). The other remarkable fact about PDI is its uncanny ability to bind things. Ligands range from peptide/protein substrates to hormones and calcium. Peptide/protein interactions are essential for effective catalysis of disulfide formation and isomerization. PDI interacts covalently with substrate cysteines (9Walker K.W. Gilbert H.F. J. Biol. Chem. 1997; 272: 8845-8848Abstract Full Text Full Text PDF PubMed Scopus (105) Google Scholar), but it also binds a variety of peptide/protein substrates (10Klappa P. Hawkins H.C. Freedman R.B. Eur. J. Biochem. 1997; 248: 37-42Crossref PubMed Scopus (84) Google Scholar). There are few strict sequence/structure requirements for binding (11Morjana N.A. Gilbert H.F. Biochemistry. 1991; 30: 4985-4990Crossref PubMed Scopus (79) Google Scholar, 12Noiva R. Freedman R.B. Lennarz W.J. J. Biol. Chem. 1993; 268: 19210-19217Abstract Full Text PDF PubMed Google Scholar), although there is some effect of sequence on reactivity with PDI (13Westphal V. Spetzler J.C. Meldal M. Christensen U. Winther J.R. J. Biol. Chem. 1998; 273: 24992-24999Abstract Full Text Full Text PDF PubMed Scopus (21) Google Scholar). PDI activity can be inhibited by a number of peptides (11Morjana N.A. Gilbert H.F. Biochemistry. 1991; 30: 4985-4990Crossref PubMed Scopus (79) Google Scholar), including the cyclic peptide antibiotic, bacitracin (14Roth R.A. Biochem. Biophys. Res. Commun. 1981; 98: 431-438Crossref PubMed Scopus (93) Google Scholar,15Medina V. Kesner L. Stracher A. Biochem. Med. Metab. Biol. 1993; 49: 255-264Crossref PubMed Scopus (8) Google Scholar). A tripeptide affinity probe, which originally identified PDI as a glycosylation site binding protein (16Geetha-Habib M. Noiva R. Kaplan H.A. Lennarz W.J. Cell. 1988; 54: 1053-1060Abstract Full Text PDF PubMed Scopus (92) Google Scholar) localized one peptide binding site near the C terminus of PDI (12Noiva R. Freedman R.B. Lennarz W.J. J. Biol. Chem. 1993; 268: 19210-19217Abstract Full Text PDF PubMed Google Scholar), but deletion of the C-terminal binding site has little effect on the oxidase and isomerase activities (17Dai Y. Wang C. J. Biol. Chem. 1997; 272: 27572-27576Abstract Full Text Full Text PDF PubMed Scopus (42) Google Scholar). Binding of radiolabeled peptides to various fragments of PDI has recently suggested that multiple sites exist that may interact with various peptides/proteins (10Klappa P. Hawkins H.C. Freedman R.B. Eur. J. Biochem. 1997; 248: 37-42Crossref PubMed Scopus (84) Google Scholar) and that a distinct structural domain (b′) that is N-terminal to one of the two active sites contributes significantly to peptide binding. Noncovalent interactions with protein substrates can suppress substrate aggregation during protein refolding. The chaperone activity of PDI is observed with proteins that form disulfides (18Puig A. Gilbert H.F. J. Biol. Chem. 1994; 269: 7764-7771Abstract Full Text PDF PubMed Google Scholar) and proteins that do not (19Wang C.C. Tsou C.L. FASEB J. 1993; 7: 1515-1517Crossref PubMed Scopus (141) Google Scholar, 20Primm T.P. Walker K.W. Gilbert H.F. J. Biol. Chem. 1996; 271: 33664-33669Abstract Full Text Full Text PDF PubMed Scopus (86) Google Scholar, 21Song J.L. Wang C.C. Eur. J. Biochem. 1995; 231: 312-316Crossref PubMed Scopus (136) Google Scholar). By interacting with multiple sites on aggregation-prone substrates, PDI can facilitate substrate aggregation under certain conditions, a behavior we have termed anti-chaperone activity (18Puig A. Gilbert H.F. J. Biol. Chem. 1994; 269: 7764-7771Abstract Full Text PDF PubMed Google Scholar, 20Primm T.P. Walker K.W. Gilbert H.F. J. Biol. Chem. 1996; 271: 33664-33669Abstract Full Text Full Text PDF PubMed Scopus (86) Google Scholar,22Puig A. Lyles M.M. Noiva R. Gilbert H.F. J. Biol. Chem. 1994; 269: 19128-19135Abstract Full Text PDF PubMed Google Scholar). In addition to unfolded protein substrates, PDI associates tightly with some folded proteins. It serves as the β subunit of prolyl 4-hydroxylase (23Koivu J. Myllyla R. Helaakoski T. Pihlajaniemi T. Tasanen K. Kivirikko K.I. J. Biol. Chem. 1987; 262: 6447-6449Abstract Full Text PDF PubMed Google Scholar) and as a subunit of the microsomal triglyceride-transfer complex (24Wetterau J.R. Combs K.A. McLean L.R. Spinner S.N. Aggerbeck L.P. Biochemistry. 1991; 30: 9728-9735Crossref PubMed Scopus (155) Google Scholar). The level of PDI present in the ER is considerably higher than either of these complexes, suggesting that PDI is involved in the folding, stability, or assembly of the complex and in its retention in the ER through the C-terminal KDEL signal of PDI (25Kivirikko K.I. Myllyla R. Pihlajaniemi T. FASEB J. 1989; 3: 1609-1617Crossref PubMed Scopus (257) Google Scholar). In addition to peptides and proteins, PDI binds hormones. It was identified by photoaffinity labeling as a thyroid-hormone binding protein (26Kitagawa S. Obata T. Hasumura S. Pastan I. Cheng S.Y. J. Biol. Chem. 1987; 262: 3903-3908Abstract Full Text PDF PubMed Google Scholar). Displacement of radiolabeled T3 from PDI purified from liver suggested a high affinity for T3 (27Horiuchi R. Yamauchi K. Hayashi H. Koya S. Takeuchi Y. Kato K. Kobayashi M. Takikawa H. Eur. J. Biochem. 1989; 183: 529-538Crossref PubMed Scopus (24) Google Scholar). However, Guthapfel et al. (28Guthapfel R. Gueguen P. Quemeneur E. Eur. J. Biochem. 1996; 242: 315-319Crossref PubMed Scopus (19) Google Scholar) have recently shown that the high affinity binding of T3 (Kd = 21 nm) has very low capacity (0.002 mol of T3/mol of PDI), implying that high affinity binding is due to an impurity rather than PDI itself. PDI also binds β-estradiol (E2), and although the number of estrogen binding sites has not been determined, estrogen has been reported to inhibit isomerase activity (29Tsibris J.C. Hunt L.T. Ballejo G. Barker W.C. Toney L.J. Spellacy W.N. J. Biol. Chem. 1989; 264: 13967-13970Abstract Full Text PDF PubMed Google Scholar). A short segment of the PDI sequence exhibits some similarity to the estrogen receptor, but this sequence does not appear to constitute an independent structural domain (30Kemmink J. Darby N.J. Dijkstra K. Scheek R.M. Creighton T.E. Protein Sci. 1995; 4: 2587-2593Crossref PubMed Scopus (47) Google Scholar). Finally, like many ER-resident proteins, PDI binds calcium with low affinity (2–5 mm) and high capacity (19 mol Ca2+/mol protein) (31Lebeche D. Lucero H.A. Kaminer B. Biochem. Biophys. Res. Commun. 1994; 202: 556-561Crossref PubMed Scopus (42) Google Scholar). Calcium affects the chaperone activity of PDI (20Primm T.P. Walker K.W. Gilbert H.F. J. Biol. Chem. 1996; 271: 33664-33669Abstract Full Text Full Text PDF PubMed Scopus (86) Google Scholar) but has no influence on the isomerase function (20Primm T.P. Walker K.W. Gilbert H.F. J. Biol. Chem. 1996; 271: 33664-33669Abstract Full Text Full Text PDF PubMed Scopus (86) Google Scholar, 33Zapun A. Creighton T.E. Rowling P.J. Freedman R.B. Proteins. 1992; 14: 10-15Crossref PubMed Scopus (52) Google Scholar). The relationships between the hormone and peptide/protein binding sites are not at all clear. The interactions of PDI with hormone ligands might be due to specific binding sites, or it may be a reflection of nonspecific binding site(s) that are normally involved in PDI/substrate interactions. There have been no systematic attempts to determine the number of binding sites for individual ligands, nor have there been any attempts to examine overlap in the interaction sites. We have used competitive ligand binding of 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid (bis-ANS), radiolabeled E2, and T3 along with various unlabeled peptide inhibitors to probe the various interactions these ligands make with PDI. The results show that PDI has multiple binding sites for hormones. Binding occurs at two different hormone binding sites. Both sites bind T3, whereas only one of them interacts with estradiol. These sites are distinct from peptide/protein binding sites. Thus, the hormone binding capacity of PDI appears to be a discrete function of the molecule and not coupled to its catalytic activity in disulfide isomerization. Because of its high local concentration in the ER, PDI could serve as an intracellular reservoir for these hormones despite its relatively low affinity. Based on the relative concentrations and affinities of PDI and the estrogen receptor, more than 90% of the intracellular T3 and estradiol would be expected to be bound to PDI. This high capacity intracellular reservoir would tend to raise the cellular levels of hormone that are necessary to cause receptor occupancy, even when the estrogen and T3 receptors have nm affinity for hormones. Recombinant rat PDI was expressed and purified as described previously (34Gilbert H.F. Kruzel M.L. Lyles M.M. Harper J.W. Protein Expression Purif. 1991; 2: 194-198Crossref PubMed Scopus (31) Google Scholar). The concentration of PDI monomers (55 kDa) was determined by absorbance at 280 nm (E0.1%= 0.94 cm−1 ml/mg) (35Gilbert H.F. Biochemistry. 1989; 29: 7298-7305Crossref Scopus (50) Google Scholar). bis-ANS was obtained from Molecular Probes, and its concentration was determined by absorbance at 385 nm using an extinction coefficient of 16,790 cm−1m−1 (36Farris F.J. Weber G. Chiang C.C. Paul I.C. J. Am. Chem. Soc. 1978; 100: 4469-4474Crossref Scopus (81) Google Scholar). E2, T3, DTT, HEPES, equine liver alcohol dehydrogenase (ADH), bacitracin, insulin chain B (oxidized, sulfonated), calcium chloride, and magnesium chloride were purchased from Sigma. ADH concentration was determined by absorbance at 280 nm using an extinction coefficient of 0.455 cm−1 ml/mg (37Cannon D.J. McKay R.H. Biochem. Biophys. Res. Commun. 1969; 35: 403-409Crossref PubMed Scopus (26) Google Scholar). Bacitracin is a mixture of multiple peptides, with the prominent species being bacitracin-A (38Siegel M.M. Huang J. Lin B. Tsao R. Edmonds C.G. Biol. Mass. Spectrom. 1994; 23: 186-204Crossref PubMed Scopus (34) Google Scholar, 39Ikai Y. Oka H. Hayakawa J. Matsumoto M. Saito M. Harada K. Mayumi Y. Suzuki M. J. Antibiot. 1995; 48: 233-242Crossref PubMed Scopus (44) Google Scholar). The molecular weight of bacitracin-A (1421) was used for estimating bacitracin concentration. Tritiated E2 (2,4,6,7-[3H]E2; specific activity, 84 Ci/mmol) and labeled T3(l-5-[125I]T3, 230 Ci/mmol) were from PerkinElmer Life Sciences with purity checked by reverse phase high pressure liquid chromatography. Measurements were made in 50 mm HEPES-NaOH, pH 7.0, containing 10 mm DTT at 25 °C using various concentrations of PDI and bis-ANS. The excitation wavelength was 370 nm, and emission was scanned from 425 to 575 nm using a PerkinElmer LS-5 fluorescence spectrometer. ADH aggregation upon thermal denaturation was followed by measuring the apparent absorbance at 360 nm caused by light scattering because of increasing turbidity (20Primm T.P. Walker K.W. Gilbert H.F. J. Biol. Chem. 1996; 271: 33664-33669Abstract Full Text Full Text PDF PubMed Scopus (86) Google Scholar). Temperature was controlled at 40 °C using a circulating water bath connected to a Beckman DU 7500 spectrophotometer. Initiation of the assay was by addition of ADH (final concentration, 11 μmdimer) to a 50 mm HEPES-NaOH buffer, pH 7.0, containing 10 mm DTT and PDI at various concentrations. The apparent velocity of aggregation (A360/min) was measured after any lag as the maximum change in absorbance with time. Oxidative refolding of RNase in the presence and absence of inhibitors was followed by an established continuous assay (8Lyles M.M. Gilbert H.F. Biochemistry. 1991; 30: 613-619Crossref PubMed Scopus (349) Google Scholar) where the formation of native RNase was measured spectrophotometrically by monitoring hydrolysis of cCMP, an RNase substrate, at 296 nm. Reduced, denatured RNase (final concentration, 8 μm) was added to PDI (1.4 μm) in a buffer containing 4.5 mm cCMP, 1 mm glutathione, 0.2 mm glutathione disulfide, 2 mm EDTA, 100 mm Tris-HCl, pH 8. RNase was reduced and denatured by overnight incubation at 10 mg/ml in denaturing buffer (140 mm DTT, 2 mm EDTA, 6 m GdnHCl, 100 mm Tris-HCl, pH 8). Denaturing buffer was exchanged for 0.1% acetic acid using a Bio-Gel P6 spin column. Reduced, denatured RNase concentration was determined by absorbance at 280 nm using an extinction coefficient of 9300 cm−1m−1. Microdialysis cells from Bel-Art Products were used with two 100 μl cavities separated by 12–14 kDa molecular mass cut-off dialysis membrane (Spectra/Por). Experiments were performed at 25 °C and incubated for 18–24 h, sufficient time to ensure equilibrium as determined by following the time course for approach to equilibrium in control experiments. Dialysis buffer contained 10 mm DTT, 10 mmNaCl, and 50 mm HEPES-NaOH, pH 7.0. Bis-ANS concentration after equilibrium dialysis was determined by absorbance at 385 nm. [3H]E2 and 125[I]T3concentrations were determined by scintillation counting calibrated against standard concentrations of [3H]E2 and125[I]T3. There was no detectable degradation of PDI during equilibration as detected by SDS-polyacrylamide gel electrophoresis analysis. PDI binds a diverse set of ligands (Fig.1), interacting with unfolded and folded proteins, E2, T3, peptide-like antibiotics such as bacitracin, and peptides such as oxidized insulin chain B (InB). Despite this promiscuity in interacting with potential ligands, the relationships among these sites have not previously been determined. PDI has been shown to bind hydrophobic ligands such as β-estradiol (27Horiuchi R. Yamauchi K. Hayashi H. Koya S. Takeuchi Y. Kato K. Kobayashi M. Takikawa H. Eur. J. Biochem. 1989; 183: 529-538Crossref PubMed Scopus (24) Google Scholar) and T3 (28Guthapfel R. Gueguen P. Quemeneur E. Eur. J. Biochem. 1996; 242: 315-319Crossref PubMed Scopus (19) Google Scholar); however, the numbers of E2 and T3 binding sites have not been determined. Equilibrium dialysis using purified, recombinant rat PDI (Fig.2) shows that there is a single E2 binding site but two T3 sites, both of which have modest affinity (Table I). Individual Kd values cannot be resolved for the two T3 binding sites, suggesting that the Kd values are similar.Figure 2Binding of T3 and β-estradiol to PDI. A, estradiol. 2 μm PDI was incubated with 2,4,6,7-3H(N)-estradiol in 50 mm HEPES, pH 7.0, 10 mm NaCl, and 10 mm DTT at 25 °C. The binding is characterized by a Kd of 2.1 ± 0.5 μm with a maximum occupancy of 0.8 ± 0.1 sites (solid curve). B, T3. PDI (4 μm) was equilibrated with [125I]T3 50 mm HEPES, pH 7.0, 10 mm NaCl, and 10 mm DTT at 25 °C. The binding is characterized by a Kd of 4.3 ± 1.4 μm with a maximum occupancy of 2.3 ± 0.2 sites (solid curve).View Large Image Figure ViewerDownload Hi-res image Download (PPT)Table IBinding of hydrophobic ligands to PDILigandKdNumber of sitesLigands that displaceμmE22.1 ± 0.50.8 ± 0.1T3T34.3 ± 1.42.3 ± 0.2bis-ANS, E2bis-ANS (fluorescence)3.0 ± 0.30.9 ± 0.2T3 Open table in a new tab Nonspecific, hydrophobic surfaces and/or binding sites can often be detected by fluorescence changes on binding the environmentally sensitive fluorescent probe, bis-ANS (40Shi L. Palleros D.R. Fink A.L. Biochemistry. 1994; 33: 7536-7546Crossref PubMed Scopus (128) Google Scholar, 41Seale J.W. Martinez J.L. Horowitz P.M. Biochemistry. 1995; 34: 7443-7449Crossref PubMed Scopus (37) Google Scholar). When bound to hydrophobic sites, bis-ANS exhibits enhanced fluorescence and a characteristic blue shift in the emission maximum. PDI binds bis-ANS with μm affinity, and the fluorescence of the bound probe is enhanced and blue-shifted (∼30 nm shift in maximum emission), indicating that the bis-ANS is binding to a hydrophobic site(s) on the protein (Fig.3 and Table I). The fluorescence intensity of bis-ANS bound to PDI was determined by titrating a limited amount of bis-ANS (0.5 μm) with increasing concentrations of PDI (0–20 μm) (40Shi L. Palleros D.R. Fink A.L. Biochemistry. 1994; 33: 7536-7546Crossref PubMed Scopus (128) Google Scholar). When calibrated in this way, the fluorescence intensity observed at excess, saturating bis-ANS suggests the binding of 0.9 ± 0.2 mol of bis-ANS/mol of PDI (Table I). Calcium, yet another PDI ligand (31Lebeche D. Lucero H.A. Kaminer B. Biochem. Biophys. Res. Commun. 1994; 202: 556-561Crossref PubMed Scopus (42) Google Scholar), enhances the fluorescence of PDI-bound bis-ANS by ∼1.6-fold with half-maximal enhancement occurring at 0.9 ± 0.1 mm calcium (data not shown). Magnesium, which like calcium affects PDI-chaperone/anti-chaperone activity, has a similar effect, but sodium chloride does not. Competition experiments show that T3 inhibits bis-ANS binding (Fig. 4). The maximum concentration of T3 is limited by solubility; however, extrapolation by fitting the data to a hyperbola suggests that T3 displaces (>87%) bis-ANS fluorescence with half-maximal inhibition occurring at 69 ± 5 μm at a bis-ANS concentration of 10 μm. None of the other known PDI ligands compete for this site; neither E2 (up to 64 μm), InB (up to 250 μm) nor bacitracin (up to 500 μm) significantly affects bis-ANS binding to PDI. Thus, PDI has one hydrophobic site that binds bis-ANS or T3, and the other ligands must bind at different sites. Equilibrium dialysis shows that there are two T3 binding sites (Fig. 2). Although one of these also binds bis-ANS, the other accommodates E2. Of all the ligands tested, only T3 affects E2 binding (Fig.5). Half-maximal inhibition occurs at 8.5 ± 3.2 μm T3, and T3 can displace E2 completely (≤95%). By contrast, neither bis-ANS, bacitracin, nor InB has any effect on E2 binding. T3 displaces both E2 and bis-ANS from their binding sites, but E2 and bis-ANS do not compete with each other. This also implies that there must be at least two independent binding sites for T3, consistent with the observed stoichiometry of T3 binding. E2 inhibits the PDI-catalyzed refolding of reduced RNase; however, the inhibition in only partial, and saturating E2 inhibits the isomerase activity by only half (Fig. 6). Although T3and E2 bind to the same site, T3 has no significant effect on PDI activity up to a concentration of 70 μm. Guthapfel et al. (28Guthapfel R. Gueguen P. Quemeneur E. Eur. J. Biochem. 1996; 242: 315-319Crossref PubMed Scopus (19) Google Scholar) reported that T3 also partially inhibits PDI with a Ki of 1.3 μm; however, under very similar conditions, we repeatedly failed to observe significant (±10%) inhibition of the isomerase activity. InB has previously been shown to inhibit the isomerase activity with an inhibition constant of 230 ± 40 μm (11Morjana N.A. Gilbert H.F. Biochemistry. 1991; 30: 4985-4990Crossref PubMed Scopus (79) Google Scholar). Bacitracin, at concentrations that have previously been reported to inhibit PDI (42Mizunaga T. Katakura Y. Miura T. Maruyama Y. J. Biochem. (Tokyo). 1990; 108: 846-851Crossref PubMed Scopus (75) Google Scholar), does inhibit PDI-catalyzed RNase refolding. However, at the same concentrations bacitracin also inhibits spontaneous RNase A refolding in the absence of PDI to the same extent (data not shown). The effects of bacitracin on PDI activity may reflect its interactions with the RNase substrate and/or its interaction with PDI. At high concentrations, PDI inhibits the aggregation of unfolded proteins. This chaperone activity, which has been attributed to the interaction of PDI and the unfolded protein substrate (8Lyles M.M. Gilbert H.F. Biochemistry. 1991; 30: 613-619Crossref PubMed Scopus (349) Google Scholar, 19Wang C.C. Tsou C.L. FASEB J. 1993; 7: 1515-1517Crossref PubMed Scopus (141) Google Scholar, 43Quan H. Fan G. Wang C.C. J. Biol. Chem. 1995; 270: 17078-17080Abstract Full Text Full Text PDF PubMed Scopus (122) Google Scholar), is observed during the folding of proteins that have disulfides as well as proteins that do not form disulfides. Under certain conditions and with certain substrate proteins, PDI exhibits what has been termed anti-chaperone activity. When it functions in its anti-chaperone mode, PDI facilitates rather than inhibits substrate aggregation (18Puig A. Gilbert H.F. J. Biol. Chem. 1994; 269: 7764-7771Abstract Full Text PDF PubMed Google Scholar, 20Primm T.P. Walker K.W. Gilbert H.F. J. Biol. Chem. 1996; 271: 33664-33669Abstract Full Text Full Text PDF PubMed Scopus (86) Google Scholar). We have suggested that the chaperone/anti-chaperone functions of PDI result from multiple, noncovalent interactions between PDI and an aggregation-prone substrate (20Primm T.P. Walker K.W. Gilbert H.F. J. Biol. Chem. 1996; 271: 33664-33669Abstract Full Text Full Text PDF PubMed Scopus (86) Google Scholar). These interactions cross-link substrate aggregates until the PDI concentration is sufficiently high to inhibit cross-linking, much like the inhibition of classical immunoprecipitation under conditions of antibody excess. Both chaperone and anti-chaperone activity are observed during the thermal aggregation of ADH (20Primm T.P. Walker K.W. Gilbert H.F. J. Biol. Chem. 1996; 271: 33664-33669Abstract Full Text Full Text PDF PubMed Scopus (86) Google Scholar). At lower concentrations of PDI, increasing PDI enhances substrate aggregation, but at higher PDI concentrations, increasing PDI inhibits substrate aggregation. At concentrations that should result in significant occupancy of their binding sites, neither E2 nor T3 have significant effects on the chaperone or anti-chaperone activity (Fig.7). These ligands also have no effect on PDI chaperone activity using citrate synthase as a substrate (20Primm T.P. Walker K.W. Gilbert H.F. J. Biol. Chem. 1996; 271: 33664-33669Abstract Full Text Full Text PDF PubMed Scopus (86) Google Scholar) (data not shown). Both InB and bis-ANS inhibit ADH aggregation in the absence of PDI, so that their effect on PDI chaperone and anti-chaperone activities could not be determined. By contrast, relatively low concentrations of bacitracin inhibited the chaperone activity without having a large effect on substrate aggregation in the absence of PDI (Fig. 7). PDI has been repeatedly isolated from cells based on its propensity to bind a surprisingly diverse group of ligands. It is the β-subunit of prolyl hydroxylase (23Koivu J. Myllyla R. Helaakoski T. Pihlajaniemi T. Tasanen K. Kivirikko K.I. J. Biol. Chem. 1987; 262: 6447-6449Abstract Full Text PDF PubMed Google Scholar) and a subunit of an ER triglyceride transferase (24Wetterau J.R. Combs K.A. McLean L.R. Spinner S.N. Aggerbeck L.P. Biochemistry. 1991; 30: 9728-9735Crossref PubMed Scopus (155) Google Scholar), although its catalytic activity is not required for the function of these complexes (25Kivirikko K.I. Myllyla R. Pihlajaniemi T. FASEB J. 1989; 3: 1609-1617Crossref PubMed Scopus (257) Google Scholar). PDI was also identified in a screen for proteins that interact with a potential glycosylation binding site present on a small peptide photo-affinity label (44LaMantia M. Miura T. Tachikawa H. Kaplan H.A. Lennarz W.J. Mizunaga T. Proc. Natl. Acad. Sci. U. S. A. 1991; 88: 4453-4457Crossref PubMed Scopus (118) Google Scholar), although it was subsequently found that PDI does not participate directly in glycosylation (45Noiva R. Kimura H. Roos J. Lennarz W.J. J. Biol. Chem. 1991; 266: 19645-19649Abstract Full Text PDF PubMed Google Scholar). In a search for thyroid hormone binding proteins, PDI was identified through photoaffinity labeling as a major T3 binding protein (46Obata T. Kitagawa S. Gong Q., H. Pastan I. Cheng S.Y. J. Biol. Chem. 1988; 263: 782-785Abstract Full Text PDF PubMed Google Scholar). PDI, like some other folding assistants (47Blond-Elguindi S. Cwirla S.E. Dower W.J. Lipshutz R.J. Sprang S.R. Sambrook J.F. Gething M.J. Cell. 1993; 75: 717-728Abstract Full Text PDF PubMed Scopus (565) Google Scholar), must interact with a large number of different substrates with few recognizable sequence cues except for a small preference for peptides that contain cysteine (10Klappa P. Hawkins H.C. Freedman R.B. Eur. J. Biochem. 1997; 248: 37-42Crossref PubMed Scopus (84) Google Scholar, 11Morjana N.A. Gilbert H.F. Biochemistry. 1991; 30: 4985-4990Crossref PubMed Scopus (79) Google Scholar). Recently, Winther and colleagues (13Westphal V. Spetzler J.C. Meldal M. Christensen U. Winther J.R. J. Biol. Chem. 1998; 273: 24992-24999Abstract Full Text Full Text PDF PubMed Scopus (21) Google Scholar) found that peptide sequence can affect the rate at which PDI reacts with peptide disulfides with some preference for a sequence that has the features, small/helix breaker-Cys-X-hydrophobic/basic-hydrophobic. Peptide binding sites have been difficult to localize on PDI. Early indications with a photo-affinity label suggested that a peptide binding site was located near the C terminus (12Noiva R. Freedman R.B. Lennarz W.J. J. Biol. Chem. 1993; 268: 19210-19217Abstract Full Text PDF PubMed Google Scholar). Deletion of this region has little effect on isomerase activity, but it does eliminate chaperone activity (17Dai Y. Wang C. J. Biol. Chem. 1997; 272: 27572-27576Abstract Full Text Full Text PDF PubMed Scopus (42) Google Scholar). Klappa et al. (10Klappa P. Hawkins H.C. Freedman R.B. Eur. J. Biochem. 1997; 248: 37-42Crossref PubMed Scopus (84) Google Scholar) have recently found that interaction between PDI and peptides involves a single domain (b′), but other sites are involved in the binding of large, protein substrates. Thus, multiple sites with limited specificity are involved in the interaction of PDI with its protein substrates. The competition binding experiments reported here show that PDI has multiple ligand binding sites (Fig. 8), some of which display overlapping specificity. However, the sites involved in hormone binding are distinct from those involved in peptide and substrate binding. Peptides and peptide analogs do not displace hormones from their binding sites, and the binding of hormones is noncompetitive with protein substrate. The partial inhibition by E2 means that protein substrates can still interact with PDI, even when E2 is bound. The lack of an observable effect on chaperone/anti-chaperone behavior also argues that the hormone sites are not utilized during the interaction between PDI and its unfolded protein substrates. Previous measurements of T3 and E2 binding to PDI have been performed by displacement of a small amount of labeled hormone by unlabeled competitor. Affinities estimated in this way suggested that PDI binds both hormones with nm affinity (26Kitagawa S. Obata T. Hasumura S. Pastan I. Cheng S.Y. J. Biol. Chem. 1987; 262: 3903-3908Abstract Full Text PDF PubMed Google Scholar, 29Tsibris J.C. Hunt L.T. Ballejo G. Barker W.C. Toney L.J. Spellacy W.N. J. Biol. Chem. 1989; 264: 13967-13970Abstract Full Text PDF PubMed Google Scholar), although lower affinity sites were observed as backgrounds. Guthapfel et al. (28Guthapfel R. Gueguen P. Quemeneur E. Eur. J. Biochem. 1996; 242: 315-319Crossref PubMed Scopus (19) Google Scholar) has recently shown that high affinity (nm) T3 binding is not stoichiometric (<0.01 mol hormone/mol PDI), implying that tight binding is from impurities rather than from PDI itself. By contrast, the low affinity (μm) binding we observe for T3 and E2 (Table I) is stoichiometric. Although PDI does not exhibit high affinity for these hormone ligands, it does have a limited number of reasonably specific sites for their interaction that do not interfere significantly with the catalytic or chaperone activities of the molecule. Thus, hormones are not binding to sites that interact with protein substrates. With dissociation constants of 2–4 μm (Table I), it would be tempting to conclude that hormone binding by PDI does not have any physiological relevance because the Kd values for hormone binding to their cellular receptors are in the low pm range (Kd = 10–300 pm) (48Neff S Sadowski C Miksicek RJ. Mol. Endocrinol. 1994; 8: 1215-1223PubMed Google Scholar, 49Walent J.H. Gorski J. Endocrinology. 1990; 126: 2383-2391Crossref PubMed Scopus (14) Google Scholar, 50Danel L. Cordier G. Revillard J.P. Saez S. Cancer Res. 1982; 42: 4701-4705PubMed Google Scholar). However, the local concentration of PDI in the ER is quite high, estimated at ∼200 μm (51Lyles M.M. Gilbert H.F. Biochemistry. 1991; 30: 619-625Crossref PubMed Scopus (118) Google Scholar). Thus, the binding of hormone to PDI will be driven by the high, local concentration of PDI despite the low Kd value. A specific example illustrates how the presence of a high capacity, low affinity reservoir will affect the location of hormone (Fig.9). With a Kd for hormone binding to PDI of 2 μm and a concentration of 200 μm PDI, simple equilibrium binding tells us that ratio [HR-PDI]/[HR]free will be 100, and 99% of the E2 in the endoplasmic reticulum will be bound to PDI. If the ER represents 10% of the cell volume, then about 90% of the intracellular E2 would be bound to PDI at any given free concentration of hormone. At a free cytosolic concentration of hormone (10 pm) that is sufficient to half-saturate the estrogen receptor, hormone binding to PDI would require a total intracellular concentration of 100 pm to generate a free hormone concentration of 10 pm. The total concentration of hormone bound to PDI would be ∼90 pm, whereas the free concentration of hormone would be 10 pm. With a high capacity reservoir for hormone binding, the concentration of hormone necessary to generate a physiological response would be significantly higher than the Kd for equilibrium hormone binding. Physiological concentrations of T3 range from about 10 pm in serum (52Butler J, Moore P, Mieli-Vergani G, Moniz C. (1988) Ann. Clin. Biochem. 25536–539Google Scholar) to 75 pm in brain cytosol (53Ferreiro B. Pastor R. Bernal J. Acta Endocrinol. 1990; 123: 95-99Crossref PubMed Scopus (26) Google Scholar). The physiological E2 concentrations are larger, from 0.2–2 nmin human serum (54Gabrielsson J. Wallenbeck I. Birgerson L. Acta Obstet. Gynecol. Scand. Supp. 1996; 163: 26-31PubMed Google Scholar), to 0.4 μm in placenta (55Nachtigall L. Bassett M. Hogsander U. Levitz M. Am. J. Obstet. Gynecol. 1968; 101: 638-648Abstract Full Text PDF Scopus (66) Google Scholar), and up to 3–5 μm in developing follicles in the ovary (56Erickson G.F. Semin. Reprod. Med. 1986; 4: 233-254Crossref Scopus (81) Google Scholar). All of these are significantly higher than the Kd measured in vitro (48Neff S Sadowski C Miksicek RJ. Mol. Endocrinol. 1994; 8: 1215-1223PubMed Google Scholar, 49Walent J.H. Gorski J. Endocrinology. 1990; 126: 2383-2391Crossref PubMed Scopus (14) Google Scholar, 50Danel L. Cordier G. Revillard J.P. Saez S. Cancer Res. 1982; 42: 4701-4705PubMed Google Scholar), suggesting that there must be some mechanism for modulating the response of hormone receptor to physiological concentrations of hormone. The function of high capacity reservoirs of low affinity may be to balance the need for high specificity and regulation. Tight binding is essential for high specificity and for discriminating between similar structures. For very high affinity receptors, such as the estrogen and T3 receptors, cell volume may limit the number of molecules present in a given cell. For example, with a cell volume of 6000 μ3 (32Korchev Y.E. Gorelik J. Lab M.J. Sviderskaya E.V Johnston C.L. Coombes C.R. Vodyanoy I. Edwards C.R. Biophys. J. 2000; 78: 451-457Abstract Full Text Full Text PDF PubMed Scopus (135) Google Scholar), at a concentration of 10 pm, there would be only 40 molecules of hormone/cell. A stochastic fluctuation in the distribution of hormone molecules in the cells would raise the possibility that some cells would not have any hormone molecules. By increasing the number of molecules in the cell by 10-fold or more, a low affinity, high capacity reservoir of hormone binding could significantly increase the number of hormone molecules/cell and decrease the probability that a cell would, by chance, find itself without hormone when a response was warranted. We thank Dr. John Putkey for use of his fluorimeter and advice and Jeff Lawton for assistance with scintillation counting.
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