Encapsulation of Human Islets in Novel Inhomogeneous Alginate-Ca 2+ /Ba 2+ Microbeads: In Vitro and In Vivo Function
2008; Marcel Dekker; Volume: 36; Issue: 5 Linguagem: Inglês
10.1080/10731190802369755
ISSN1532-4184
AutoresMeirigeng Qi, Berit L. Strand, Ýrr Mørch, Igor Lacı́k, Yong Wang, Payam Salehi, Barbara Barbaro, Antonio Gangemi, Joseph B. Kuechle, Travis Romagnoli, Michael A. Hansen, L Rodriguez, Enrico Benedetti, David Hunkeler, Gudmund Skjåk‐Bræk, José Oberholzer,
Tópico(s)Diabetes and associated disorders
ResumoMicroencapsulation may allow for immunosuppression-free islet transplantation. Herein we investigated whether human islets can be shipped safely to a remote encapsulation core facility and maintain in vitro and in vivo functionality. In non-encapsulated islets before and encapsulated islets after shipment, viability was 88.3±2.5 and 87.5±2.7% (n=6, p=0.30). Stimulation index after static glucose incubation was 5.4±0.5 and 6.3±0.4 (n=6, p=0.18), respectively. After intraperitoneal transplantation, long-term normoglycemia was consistently achieved with 3,000, 5,000, and 10,000 IEQ encapsulated human islets. When transplanting 1,000 IEQ, mice returned to hyperglycemia after 30-55 (n=4/7) and 160 days (n=3/7). Transplanted mice showed human oral glucose tolerance with lower glucose levels than non-diabetic control mice. Capsules retrieved after transplantation were intact, with only minimal overgrowth. This study shows that human islets maintained the viability and in vitro function after encapsulation and the inhomogeneous alginate-Ca2+/Ba2+ microbeads allow for long-term in vivo human islet graft function, despite long-distance shipment.
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