On-column sample concentration of high-ionic-strength samples in capillary electrophoresis
1998; Wiley; Volume: 10; Issue: 6 Linguagem: Inglês
10.1002/(sici)1520-667x(1998)10
ISSN1520-667X
AutoresSangryoul Park, Craig E. Lunte,
Tópico(s)Electrowetting and Microfluidic Technologies
ResumoHigh-ionic-strength samples result in poor electrophoretic separation efficiencies due to “destacking” of the sample. A simple method is described to stack high-ionic-strength samples without matrix modification prior to injection. Using a weak-acid buffer such as acetate as the background electrolyte, the electrokinetic injection of the sample is immediately followed by electrokinetic injection of an acid solution. The resulting titration of the background electrolyte produces a low-conductivity zone across which sample cations are focused. A sevenfold sensitivity enhancement was achieved with improved separation efficiency relative to normal electrokinetic injection. © 1998 John Wiley & Sons, Inc. J Micro Sep 10: 511–517, 1998
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