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How suspension cultures of Catharanthus roseus respond to oxygen limitation: Small‐scale tests with applications to large‐scale cultures

1989; Wiley; Volume: 34; Issue: 8 Linguagem: Inglês

10.1002/bit.260340806

1097-0290

Jamie Snape, N. H. Thomas, J. A. Callow,

Enzyme Catalysis and Immobilization

The effect of oxygen supply on the growth of suspension cultures of Catharanthus roseus in Erlenmeyer flasks was investigated. Below a critical oxygen supply rate the culture could not survive. By increasing the oxygen supply, a point is reached where the culture survives but no growth is possible. At higher oxygen supply rates there is a regime where both growth rate and the maximum biomass concentration increase with oxygen supply. Eventually there comes a point where no further increase in biomass is achieved, probably due to the depletion of the sugars; however, the growth rate continues to increase with oxygen supply until a maximum growth rate is obtained. The ratio of fresh to dry weight at maximum fresh weight increased with shaker table speed of rotation accompanied by a greater rate of sugar depletion.