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The Effect of Treatment with Interleukin-1 and Tumor Necrosis Factor on Pseudomonas aeruginosa Lung Infection in a Granulocytopenic Mouse Model

1994; Academic Press; Volume: 73; Issue: 2 Linguagem: Inglês

10.1006/clin.1994.1196

1090-2341

Claudia R. Amura, Patricia A. Fontán, Norberto Sanjuán, Daniel O. Sordelli,

Inflammation biomarkers and pathways

The efficacy of treatment with interleukin-lβ (IL-1β) and tumor necrosis factor-α (TNFα) on Pseudomonas aeruginosa pneumonia was evaluated in a granulocytopenic mouse model. Combined intravenous administration of 2000 U IL-1β plus 2000 U TNFα significantly diminished mortality from aerosol challenge with P. aeruginosa. Mice treated with IL-1β, TNFα, or both also exhibited a significant enhancement in pulmonary clearance of P. aeruginosa. Combined cytokine administration induced an increase in the pulmonary content of myeloperoxidase activity. Mature leukocytes were not detected in either circulation or bronchoalveolar lavage fluid from granulocytopenic, cytokine-treated mice. In conclusion, IL-1β and TNFα, treatment exhibited a synergistic protective effect from pulmonary P. aeruginosa challenge in granulocytopenic hosts, probably due to enhancement of nonspecific antibacterial mechanisms.