Revisão Revisado por pares

Detection of telomerase activity by the TRAP assay and its variants and alternatives

2006; Elsevier BV; Volume: 371; Issue: 1-2 Linguagem: Inglês

10.1016/j.cca.2006.02.039

ISSN

1873-3492

Autores

Jiřı́ Fajkus,

Tópico(s)

Microplastics and Plastic Pollution

Resumo

Telomerase activity is closely connected to problems of cellular immortality, proliferative capacity, differentiation, cancer and aging. Correspondingly, techniques for its detection have been essential for progress in telomere biology and are of still increasing importance in molecular diagnostics and therapy of cancer. This article reviews the development of the telomere repeat amplification protocol (TRAP) and its various modifications as the most widespread assay to detect and measure telomerase activity. Alternative possibilities of telomerase activity detection are also discussed which make it possible to omit the PCR-mediated amplification of telomerase products. These approaches are based on recent advances in highly sensitive detection systems.

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