A CHEMICAL METHOD FOR THE DETERMINATION OF ACETYLCHOLINE: ITS APPLICATION IN A STUDY OF PRESYNAPTIC RELEASE AND A CHOLINE ACETYLTRANSFERASE ASSAY 1
1969; Wiley; Volume: 16; Issue: 2 Linguagem: Inglês
10.1111/j.1471-4159.1969.tb05932.x
ISSN1471-4159
Autores Tópico(s)Analytical Chemistry and Chromatography
ResumoAbstract— 1. A chemical method for the determination of acetylcholine has been devised based upon (a) The precipitation of acetylcholine out of solution as an iodine complex. (b) The absorption of the quaternary ester to a ‘carboxyl’ ion exchange resin column. (c) The hydrazinolysis of the acetylcholine to form acetyl hydrazide. (d) The formation of a fluorescent molecule with salicylaldehyde. 2. The method was utilized to determine (a) If tetramethylammonium ions would release acetylcholine from the cat cervical synaptic ganglion. (b) The activity of enzyme choline acetyltransferase in rat brain. 3. The observation that tetramethylammonium ions do release acetylcholine sustains the viability of the hypothesis that acetylcholine forms a primary acyl bond with its receptor.
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