Evidence for circulating hemocyte proliferation in the shrimp Penaeus japonicus
1996; Elsevier BV; Volume: 20; Issue: 2 Linguagem: Inglês
10.1016/0145-305x(96)00001-8
ISSN1879-0089
AutoresTeresa Sequeira, Delfina Tavares, Mário Arala‐Chaves,
Tópico(s)Aquaculture Nutrition and Growth
ResumoWe have observed by flow cytometric analysis that a small but consistent percentage of circulating hemocytes in phases S, G2 and M of the cell cycle can be detected in Penaeus japonicus. Significantly increased percentages of proliferating hemocytes (~ three-fold increase) were observed after stimulation by lipopolysaccharide (LPS), p43 (an immunosuppressive lymphocyte mitogenic protein produced by Candida albicans) and a combination of LPS and p43. Moreover, an ~ six-fold increase in the percentage of proliferating hemocytes was also observed in animals infected with Fusarium spp., as compared to non-infected shrimps. Furthermore, [3H] thymidine uptake in circulating hemocytes was 26 times greater in LPS stimulated than in non-stimulated shrimps. The present study suggests that circulating hemocytes of the shrimp P. japonicus can divide in vivo and that proliferation can be increased significantly after mitogenic or infectious stimulation.
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