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Sensitivity and specificity of serological tests, histopathology and immunohistochemistry for detection of Toxoplasma gondii infection in domestic chickens

2014; Elsevier BV; Volume: 204; Issue: 3-4 Linguagem: Inglês

10.1016/j.vetpar.2014.05.039

1873-2550

Luciana Casartelli Alves, Viviane Cardoso Boechat, R. Macedo-Couto, Luiz Cláudio Ferreira, José Leonardo Nicolau, Leandro Batista das Neves, Patrícia Riddell Millar, Regiane Trigueiro Vicente, Raquel de Vasconcellos Carvalhães de Oliveira, A.G. Muniz, Isabel Cristina Fábregas Bonna, Maria Regina Reis Amendoeira, Rodrigo Costa da Silva, Hélio Langoni, T. M. P. Schubach, Rodrigo Caldas Menezes,

Parasitic Infections and Diagnostics

Since free-range chickens are important for the epidemiology of toxoplasmosis, this study evaluated the sensitivity and specificity of different laboratory techniques for the diagnosis of Toxoplasma gondii in these animals. Serum samples from 135 adult domestic chickens were tested for anti-T. gondii antibodies by the indirect fluorescent antibody test (IFAT), enzyme-linked immunosorbent assay (ELISA), modified agglutination test (MAT), and indirect hemagglutination test (IHAT). Tissue samples from all animals were analyzed by histopathology, immunohistochemistry and mouse bioassay (gold standard). Fifty-four chickens were positive for T. gondii in the bioassay. The sensitivity and specificity of the different tests were, respectively, 85% and 56% for ELISA; 80% and 52% for IFAT; 76% and 68% for MAT; 61% and 80% for IHAT; 7% and 98% for immunohistochemistry, and 6% and 98% for histopathology. The MAT was the most effective method for the diagnosis of T. gondii infection in chickens, followed by ELISA. Histopathology and immunohistochemistry are useful tools for the diagnosis of T. gondii infection in chickens due to their specificity.