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Artigo Produção Nacional Revisado por pares
BIBTEX RIS

Induction of Partial Protection in Mice after Oral Administration of Lactococcus lactis Producing Brucella abortus L7/L12 Antigen

2003; Taylor & Francis; Volume: 11; Issue: 8-10 Linguagem: Inglês

10.1080/10611860410001670035

ISSN

1061-186X

Autores

Daniela Santos Pontes, Fernanda Alves Dorella, Luciana Ribeiro, Anderson Miyoshi, Yves Le Loir, Alexandra Gruss, Sérgio C. Oliveira, Philippe Langella, Vasco Azevedo,

Tópico(s)

Bacteriophages and microbial interactions

Resumo

AbstractThe Brucella abortus ribosomal protein L7/L12 is an immunodominant antigen and an interesting candidate for the development of oral live vaccines against brucellosis. Here, a recombinant Lactococcus lactis strain producing L7/L12 under the control of nisin inducible promoter was orally administered to BALB/c mice. Significant levels of anti-L7/L12 specific IgA detected in feces revealed an induced local humoral immune response. However, serum analysis did not reveal any anti-L7/L12 antibodies suggesting the absence of a systemic response. Nevertheless, the vaccinated mice showed a partial protective immunity against B. abortus virulent strain (S2308) challenged by intraperitoneal inoculation.Keywords:: L7/L12Live oral vaccineImmune response

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