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UNILATERAL URETERAL OBSTRUCTION INDUCES RENAL TUBULAR CELL PRODUCTION OF TUMOR NECROSIS FACTOR-α INDEPENDENT OF INFLAMMATORY CELL INFILTRATION

2004; Lippincott Williams & Wilkins; Volume: 172; Issue: 4 Part 2 Linguagem: Inglês

10.1097/01.ju.0000138902.57626.70

1527-3792

Rosalia Misseri, Daniel R. Meldrum, Pierre C. Dagher, Karen L. Hile, Richard C. Rink, Kirstan K. Meldrum,

Nanoplatforms for cancer theranostics

No AccessJournal of UrologyKidney1 Oct 2004UNILATERAL URETERAL OBSTRUCTION INDUCES RENAL TUBULAR CELL PRODUCTION OF TUMOR NECROSIS FACTOR-α INDEPENDENT OF INFLAMMATORY CELL INFILTRATION ROSALIA MISSERI, DANIEL R. MELDRUM, PIERRE DAGHER, KAREN HILE, RICHARD C. RINK, and KIRSTAN K. MELDRUM ROSALIA MISSERIROSALIA MISSERI , DANIEL R. MELDRUMDANIEL R. MELDRUM , PIERRE DAGHERPIERRE DAGHER , KAREN HILEKAREN HILE , RICHARD C. RINKRICHARD C. RINK , and KIRSTAN K. MELDRUMKIRSTAN K. MELDRUM View All Author Informationhttps://doi.org/10.1097/01.ju.0000138902.57626.70AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Obstructive uropathy is a significant clinical problem that results in apoptotic renal cell death and progressive renal fibrosis. A number of different inflammatory mediators have been implicated in the pathophysiology of obstruction induced renal injury including tumor necrosis factor-α (TNF)-α. The cellular source of obstruction induced renal TNF-α production and its relationship to renal inflammatory cell infiltration remain unknown. Materials and Methods: Male Sprague-Dawley rats were anesthetized and exposed to varying lengths of unilateral ureteral obstruction vs sham operation. The kidneys were harvested following renal injury and evaluated for TNF-α mRNA expression (reverse transcriptase polymerase chain reaction), TNF-α protein production (enzyme-linked immunosorbent assay), TNF-α cellular localization (immunohistochemistry) and leukocyte infiltration (leukocyte staining). Results: Renal TNF-α mRNA expression and protein production peaked following 3 days of ureteral obstruction (54 ± 5% vs sham 22 ± 9% of glyceraldehyde-3-phosphate dehydrogenase mRNA, p <0.05 and 204 ± 13 vs sham 84 ± 9 pg/ml, p <0.05, respectively). TNF-α production localized primarily to renal cortical tubular cells following obstruction and the time point of maximal TNF-α production (3 days of obstruction) were not associated with a significant renal inflammatory cell infiltrate. Conclusions: TNF-α is produced by the renal cortical tubular cells in response to ureteral obstruction and independent of a significant inflammatory cell infiltrate. Identification of the cellular source of TNF-α expression during renal obstruction may have therapeutic implications for the targeted inhibition of TNF-α production and potential amelioration of obstructive renal injury. 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Google Scholar From the Departments of Urology (RM, KH, RCR, KKM), Surgery (DRM) and Medicine (PD), Indiana University, Indianapolis, Indiana© 2004 by American Urological Association, Inc.FiguresReferencesRelatedDetailsCited byBani-Hani A, Campbell M, Meldrum D and Meldrum K (2008) Cytokines in Epithelial-Mesenchymal Transition: A New Insight Into Obstructive NephropathyJournal of Urology, VOL. 180, NO. 2, (461-468), Online publication date: 1-Aug-2008.Metcalfe P and Meldrum K (2018) Sex Differences and the Role of Sex Steroids in Renal InjuryJournal of Urology, VOL. 176, NO. 1, (15-21), Online publication date: 1-Jul-2006. Volume 172Issue 4 Part 2October 2004Page: 1595-1599 Advertisement Copyright & Permissions© 2004 by American Urological Association, Inc.KeywordsleukocytesinflammationcytokineskidneyMetricsAuthor Information ROSALIA MISSERI More articles by this author DANIEL R. MELDRUM More articles by this author PIERRE DAGHER More articles by this author KAREN HILE More articles by this author RICHARD C. RINK More articles by this author KIRSTAN K. MELDRUM More articles by this author Expand All Advertisement PDF downloadLoading ...