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Development of microsatellite PCR typing methodology for the sea louse, Lepeophtheirus salmonis (Krøyer)

2000; Wiley; Volume: 31; Issue: 11 Linguagem: Inglês

10.1046/j.1365-2109.2000.00514.x

1365-2109

D Nolan, Samuel Martín, Y Kelly, Kelsey L. Glennon, Roy Palmer, Terry Smith, Grace P. McCormack, R. Powell,

Identification and Quantification in Food

Aquaculture ResearchVolume 31, Issue 11 p. 815-822 Development of microsatellite PCR typing methodology for the sea louse, Lepeophtheirus salmonis (Krøyer) D V Nolan, D V Nolan Department of Microbiology andSearch for more papers by this authorS A M Martin, S A M Martin National Diagnostics Centre – BioResearch Ireland, National University of Ireland, Galway, Ireland Department of Zoology, Natural History Museum, Cromwell Road, London SW7 5BD, UKSearch for more papers by this authorY Kelly, Y Kelly National Diagnostics Centre – BioResearch Ireland, National University of Ireland, Galway, Ireland Department of Zoology, Natural History Museum, Cromwell Road, London SW7 5BD, UKSearch for more papers by this authorK Glennon, K Glennon National Diagnostics Centre – BioResearch Ireland, National University of Ireland, Galway, Ireland Department of Zoology, Natural History Museum, Cromwell Road, London SW7 5BD, UKSearch for more papers by this authorR Palmer, R Palmer National Diagnostics Centre – BioResearch Ireland, National University of Ireland, Galway, Ireland Department of Zoology, Natural History Museum, Cromwell Road, London SW7 5BD, UKSearch for more papers by this authorT Smith, T Smith National Diagnostics Centre – BioResearch Ireland, National University of Ireland, Galway, Ireland Department of Zoology, Natural History Museum, Cromwell Road, London SW7 5BD, UKSearch for more papers by this authorG P McCormack, G P McCormackSearch for more papers by this authorR Powell, R Powell Department of Microbiology andSearch for more papers by this author D V Nolan, D V Nolan Department of Microbiology andSearch for more papers by this authorS A M Martin, S A M Martin National Diagnostics Centre – BioResearch Ireland, National University of Ireland, Galway, Ireland Department of Zoology, Natural History Museum, Cromwell Road, London SW7 5BD, UKSearch for more papers by this authorY Kelly, Y Kelly National Diagnostics Centre – BioResearch Ireland, National University of Ireland, Galway, Ireland Department of Zoology, Natural History Museum, Cromwell Road, London SW7 5BD, UKSearch for more papers by this authorK Glennon, K Glennon National Diagnostics Centre – BioResearch Ireland, National University of Ireland, Galway, Ireland Department of Zoology, Natural History Museum, Cromwell Road, London SW7 5BD, UKSearch for more papers by this authorR Palmer, R Palmer National Diagnostics Centre – BioResearch Ireland, National University of Ireland, Galway, Ireland Department of Zoology, Natural History Museum, Cromwell Road, London SW7 5BD, UKSearch for more papers by this authorT Smith, T Smith National Diagnostics Centre – BioResearch Ireland, National University of Ireland, Galway, Ireland Department of Zoology, Natural History Museum, Cromwell Road, London SW7 5BD, UKSearch for more papers by this authorG P McCormack, G P McCormackSearch for more papers by this authorR Powell, R Powell Department of Microbiology andSearch for more papers by this author First published: 24 December 2001 https://doi.org/10.1046/j.1365-2109.2000.00514.xCitations: 15 R Powell, Department of Microbiology, National University of Ireland, Galway, Ireland Present addresses:Department of Zoology, University of Aberdeen, Tillydrone Avenue, Aberdeen AB24 2TZ, UK; and ‡Department of Zoology, University College Cork, Ireland Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract In order to develop a microsatellite typing system for Lepeophtheirus salmonis (Krøyer), a DNA preparation method for individual sea lice suitable for analysis by polymerase chain reaction (PCR) was designed, and the DNA sequences of 50 L. salmonis microsatellite elements were determined. The microsatellites were composed of 60% perfect, 25% imperfect, and 15% compound repeats. Based on the flanking DNA sequences, four microsatellite-PCR assays were optimized and used in a pilot study to analyse L. salmonis samples collected in Ireland, Norway and Scotland. Two of the microsatellite-PCR assays targeted polymorphic loci amplifying seven and 10 alleles respectively. The results showed that microsatellite-PCR typing could detect genetic variation both within and between the L. salmonis groups, and also was capable of amplifying group-specific alleles. 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Citing Literature Volume31, Issue11November 2000Pages 815-822 ReferencesRelatedInformation