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GPU-based image registration in aperture correlation microscopy, and reflection mode correlation microscopy

2013; SPIE; Volume: 8589; Linguagem: Inglês

10.1117/12.2004968

1996-756X

Lionel J. Fafchamps, Mark A. A. Neil, R. Juškaitis,

Integrated Circuits and Semiconductor Failure Analysis

Aperture Correlation Microscopy (ACM) is a fluorescence microscopy technique capable of depth resolved imaging and enhanced lateral resolution at real-time acquisition rates. It relies on the subtraction of 2 separate images from different cameras which must be registered to the sub-pixel level. In order to achieve real-time registration and subtraction, the graphics processing unit (GPU) is used to apply a transformation from one frame to the other, resulting in a system capable of processing over 200 frames per second on modest hardware (GeForce 330M). Currently, this rate is limited by camera acquision to 16fps. Additionally, a novel reflection mode correlation microscope is introduced which functions on similar principles as the fluorescent system but can be used to examine reflective samples. Images and z-stacks taken with this system are presented here.