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Protein kinase B/Akt phosphorylation of PDE3A and its role in mammalian oocyte maturation

2006; Springer Nature; Volume: 25; Issue: 24 Linguagem: Inglês

10.1038/sj.emboj.7601431

1460-2075

Seung Jin Han, Sergio Vaccari, Taku Nedachi, Carsten Andersen, Kristina S. Kovacina, Richard A. Roth, Marco Conti,

Genetics, Aging, and Longevity in Model Organisms

Article23 November 2006free access Protein kinase B/Akt phosphorylation of PDE3A and its role in mammalian oocyte maturation Seung Jin Han Seung Jin Han Division of Reproductive Biology, Department of Obstetrics and Gynecology, Stanford University, Stanford, CA, USARecipients of a fellowship from the Lalor Foundation Search for more papers by this author Sergio Vaccari Sergio Vaccari Division of Reproductive Biology, Department of Obstetrics and Gynecology, Stanford University, Stanford, CA, USARecipients of a fellowship from the Lalor Foundation Search for more papers by this author Taku Nedachi Taku Nedachi Tohoku University Biomedical Engineering Research Organization, Sendai, Japan Search for more papers by this author Carsten B Andersen Carsten B Andersen Genomics Institute of the Novartis Research Foundation, San Diego, CA, USA Search for more papers by this author Kristina S Kovacina Kristina S Kovacina Department of Molecular Pharmacology, Stanford University, Stanford, CA, USA Search for more papers by this author Richard A Roth Richard A Roth Department of Molecular Pharmacology, Stanford University, Stanford, CA, USA Search for more papers by this author Marco Conti Corresponding Author Marco Conti Division of Reproductive Biology, Department of Obstetrics and Gynecology, Stanford University, Stanford, CA, USA Search for more papers by this author Seung Jin Han Seung Jin Han Division of Reproductive Biology, Department of Obstetrics and Gynecology, Stanford University, Stanford, CA, USARecipients of a fellowship from the Lalor Foundation Search for more papers by this author Sergio Vaccari Sergio Vaccari Division of Reproductive Biology, Department of Obstetrics and Gynecology, Stanford University, Stanford, CA, USARecipients of a fellowship from the Lalor Foundation Search for more papers by this author Taku Nedachi Taku Nedachi Tohoku University Biomedical Engineering Research Organization, Sendai, Japan Search for more papers by this author Carsten B Andersen Carsten B Andersen Genomics Institute of the Novartis Research Foundation, San Diego, CA, USA Search for more papers by this author Kristina S Kovacina Kristina S Kovacina Department of Molecular Pharmacology, Stanford University, Stanford, CA, USA Search for more papers by this author Richard A Roth Richard A Roth Department of Molecular Pharmacology, Stanford University, Stanford, CA, USA Search for more papers by this author Marco Conti Corresponding Author Marco Conti Division of Reproductive Biology, Department of Obstetrics and Gynecology, Stanford University, Stanford, CA, USA Search for more papers by this author Author Information Seung Jin Han1,‡, Sergio Vaccari1,‡, Taku Nedachi2, Carsten B Andersen3, Kristina S Kovacina4, Richard A Roth4 and Marco Conti 1 1Division of Reproductive Biology, Department of Obstetrics and Gynecology, Stanford University, Stanford, CA, USA 2Tohoku University Biomedical Engineering Research Organization, Sendai, Japan 3Genomics Institute of the Novartis Research Foundation, San Diego, CA, USA 4Department of Molecular Pharmacology, Stanford University, Stanford, CA, USA ‡These authors contributed equally to this work *Corresponding author. Division of Reproductive Biology, Department of Obstetrics and Gynecology, Stanford University, 300 Pasteur dr., Stanford, CA 94305, USA. Tel.: +1 650 725 2452; Fax: +1 650 725 7102; E-mail: [email protected] The EMBO Journal (2006)25:5716-5725https://doi.org/10.1038/sj.emboj.7601431 Recipients of a fellowship from the Lalor Foundation PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info cGMP-inhibited cAMP phosphodiesterase 3A (PDE3A) is expressed in mouse oocytes, and its function is indispensable for meiotic maturation as demonstrated by genetic ablation. Moreover, PDE3 activity is required for insulin/insulin-like growth factor-1 stimulation of Xenopus oocyte meiotic resumption. Here, we investigated the cAMP-dependent protein kinase B (PKB)/Akt regulation of PDE3A and its impact on oocyte maturation. Cell-free incubation of recombinant mouse PDE3A with PKB/Akt or cAMP-dependent protein kinase A catalytic subunits leads to phosphorylation of the PDE3A protein. Coexpression of PDE3A with constitutively activated PKB/Akt (Myr-Akt) increases PDE activity as well as its phosphorylation state. Injection of pde3a mRNA potentiates insulin-dependent maturation of Xenopus oocytes and rescues the phenotype of pde3−/− mouse oocytes. This effect is greatly decreased by mutation of any of the PDE3A serines 290–292 to alanine in both Xenopus and mouse. Microinjection of myr-Akt in mouse oocytes causes in vitro meiotic maturation and this effect requires PDE3A. Collectively, these data indicate that activation of PDE3A by PKB/Akt-mediated phosphorylation plays a role in the control of PDE3A activity in mammalian oocytes. Introduction Mammalian and amphibian oocytes progress through the meiotic cell cycle until the prophase of meiosis I, and then are arrested at a G2-like phase. During each reproductive cycle in vivo, resumption of meiosis is triggered by the preovulatory surge of luteinizing hormone (LH). Resumption of meiosis and progression of metaphase II is characterized by germinal vesicle breakdown (GVBD), chromatin condensation and segregation, and extrusion of the first polar body. Unlike the mitotic cell cycle where DNA synthesis occurs during the S phase, oocytes immediately enter a second meiotic division without the S phase and are arrested in metaphase II. Only metaphase II stage oocytes are able to undergo fertilization by spermatozoa, complete the second meiotic maturation, and form pronuclei. Although it is well known that the LH surge triggers oocyte maturation, the mechanistic details of this regulation remain unclear. Because LH does not act directly on oocytes, intermediate steps must be present to transduce LH action to oocyte maturation. Recently, it has been proposed that the effect of LH in rodent oocytes is mediated by epidermal growth factor-like peptide hormones (EGF-like growth factors), such as epiregulin, amphiregulin, and betacellulin (Park et al, 2004), which function as paracrine signals between mural granulosa and cumulus cells (Conti et al, 2005). However, the signaling mechanisms of how EGF-like growth factors affect rodent oocyte maturation have not been defined. Although its physiological significance is debated, it has been established that insulin-like growth factor-I (IGF-I) and insulin are potent inducers of meiotic resumption in Xenopus oocytes (El-Etr et al, 1979; Maller and Koontz, 1981). IGF-I- or insulin-dependent Xenopus oocyte maturation is mediated by activation of phosphoinositide 3 (PI3)-kinase and cAMP-dependent protein kinase B (PKB)/Akt (Liu et al, 1995; Deuter-Reinhard et al, 1997). In addition, we have shown that activation of PKB/Akt is necessary and sufficient for IGF-I- or insulin-dependent maturation (Andersen et al, 1998, 2003). Thus, PI3-kinase and PKB/Akt activation play an important role in growth factor-dependent oocyte meiotic maturation. High levels of cyclic AMP (cAMP) are involved in maintaining meiotic arrest in both mammals and amphibians as well as some invertebrate species (Maller and Krebs, 1977; Meijer et al, 1989; Tsafriri et al, 1996; Conti et al, 1998). In both mammalian and amphibian oocytes, considerable evidence indicates that phosphodiesterase type III (PDE3), which hydrolyzes cAMP, plays an important role in regulating resumption of meiosis (Tsafriri et al, 1996; Shitsukawa et al, 2001; Conti et al, 2002). Of the two cGMP-inhibited cAMP phosphodiesterase 3A (PDE3A) and PDE3B isoenzymes, only PDE3A is expressed in the rodent oocyte and is responsible for the regulation of oocyte cAMP concentration (Richard et al, 2001; Shitsukawa et al, 2001). In Xenopus oocytes, insulin-dependent meiotic resumption is completely blocked by cilostamide, a potent PDE3 inhibitor, whereas rolipram, a PDE4 inhibitor, has no effect (Sadler, 1991; Andersen et al, 1998). The spontaneous maturation of rodent oocytes is also blocked by cilostamide treatment and more importantly, the pde3a null female mice are sterile because of a meiotic block at the G2–M transition (Masciarelli et al, 2004). All these data suggest that PDE3A is critical for meiotic maturation. However, the regulatory mechanisms of PDE3A activity and their involvement in oocyte maturation are unclear. Here, we investigated the mechanisms of regulation of PDE3A activity and the potential role of this activity in the control of cAMP levels and GVBD in oocytes. Our data demonstrate that PDE3A is phosphorylated by PKB/Akt and that this phosphorylation plays a role during meiotic maturation induced by growth factors. Results PDE3A is phosphorylated and activated by PKB/Akt and PKA Given the observation that the paralog PDE3B is a substrate of cAMP-dependent protein kinase A (PKA) and PKB/Akt (Movsesian, 2002), we used several strategies to elucidate whether PDE3A also is phosphorylated by these kinases. Immunoprecipitated recombinant PDE3A was incubated with radiolabeled ATP in the presence or absence of purified active PKB/Akt or PKA catalytic subunit. PDE3A was phosphorylated in the presence of either kinase in this cell-free system (Figure 1A). To determine whether PDE3A is phosphorylated by PKB/Akt also in intact cells, cells expressing PDE3A were cotransfected with different Akt constructs, including wildtype (WT), kinase dead mutant (K179M) PKB/Akt (KD-Akt), constitutively active ΔPH myristoylated PKB/Akt (myr-Akt), or the form of myr-Akt (A2myr-Akt) that is modified in the myristoylation signal (Kohn et al, 1996; Figure 1B). After immunoprecipitation with HA antibodies, the phosphorylation of PDE3A was determined by SDS–PAGE and blotting with phospho-Akt substrate antibodies. Coexpression with myr-Akt caused a major increase in the phosphorylation of PDE3A, whereas minor phosphorylation with WT-Akt (Supplementary Figure 1) or no phosphorylation (KD-Akt, A2myr-Akt) was observed (Figure 1C). These results further suggest that PDE3A is a physiological substrate of PKB/Akt in the intact cells. Figure 1.PDE3A is phosphorylated by PKB/Akt and PKA. (A) Immunoprecipitated PDE3A was incubated with or without recombinant PKB/Akt or PKA in the presence of [γ-32P]ATP. The reaction products were subjected to SDS–PAGE and phosphorylated PDE3A (upper panel; pPDE3A) was detected after treatment of PKA or PKB/Akt. The amount of expressed PDE3A was monitored by Western blot analysis using anti-HA antibodies (lower panel; PDE3A). (B) Diagram of the constructs of PKB/Akt used in this study. (C) PDE3A-transfected (PDE3A) or empty vector-transfected (Mock) cells were cotransfected with different Akt constructs including WT, kinase-dead mutant (K179M) PKB/Akt (KD-Akt), constitutively active ΔPH myristoylated PKB/Akt (myr-Akt), or myr-Akt (A2myr-Akt). Immunoprecipitates were subjected to SDS–PAGE followed by Western blot analysis using phospho-Akt substrate antibodies. Coexpression with myr-Akt caused an increase in the phosphorylation of PDE3A (pPDE3A). The expression of PDE3A and various Akt constructs was confirmed by Western blot using anti-HA antibodies (PDE3A, Akt, Myr-Akt). (D) Coexpression of PDE3A with PKB/Akt enhances PDE3A activity. A first transfection (1st Tfn) with empty vector (Mock) or PDE3A-HA (PDE3A) was followed by a second transfection (2nd Tfn) using one of PKB/Akt constructs. PDE3A activity was enhanced by WT- or myr-Akt by 1.5- to 2-fold, but not by KD-Akt and A2myr-Akt. Expression of PDE3A and Akt constructs was analyzed by Western blot using anti-HA antibodies (PDE3A, Akt, Myr-Akt). A representative experiment of the three performed is reported. * and *** represent values of P<0.05 and P<0.005, respectively, compared to control. Download figure Download PowerPoint To test whether the PKB/Akt-mediated phosphorylation is associated with changes in PDE3A activity, sequential transfection was performed with PDE3A and one of the PKB/Akt constructs including WT-Akt, KD-Akt, myr-Akt, or A2myr-Akt. PDE3A was significantly activated by WT- or myr-Akt by 1.5- to 2-fold, respectively (Figure 1D, upper panel). This finding suggests that kinase activity of PKB/Akt is necessary for PDE3A activation. The amount of PDE3A expressed was not affected by myr-Akt transfection (Figure 1D, lower panel), ruling out that the increase in activity is due to increasing amounts of PDE3A protein. Because Akt-related kinase serum- and glucocorticoid-inducible kinase (Sgk) shares a similar structure with PKB/Akt (without the PH domain) and phosphorylates similar substrates (Murray et al, 2005), we tested whether this kinase has a similar effect on PDE3A. However, a constitutively active Sgk mutant did not activate PDE3A (data not shown). This set of experiments demonstrated that PDE3A is a substrate for PKB/Akt but not Sgk, even though these two kinases have a similar catalytic domain, further supporting the specificity of the Akt effects observed. Serine 290–292 phosphorylation of PDE3A is important for activation The activation of PDE3A by PKB/Akt is likely mediated by direct phosphorylation as there are at least five potential PKB/Akt phosphorylation motifs (RxRxxS/T) in the mouse PDE3A sequence (S35, S271, S291, S292, and S462; Figure 2A). An alignment search revealed that three of them, S291, S292, and S462, are conserved among mouse, human, and rat PDE3A sequences (Figure 2A; Supplementary Figure 2). To investigate whether these phosphorylation sites at the amino terminus of the protein are important for PKB/Akt-dependent activation of PDE3A, we generated a truncated form of PDE3A (ΔNcoI-PDE3A). The construct lacks the amino-terminal domain that contains all the putative phosphorylation sites with the exception of S462. This mutant was not activated by PKB/Akt, confirming that the amino-terminal region of PDE3A is important for mediating the PKB/Akt effects (Figure 2B). Figure 2.An amino-terminus deletion mutant of PDE3A is not activated by PKB/Akt. (A) Putative PKB/Akt phosphorylation sites in the PDE3A amino-acid sequence. Serines marked with asterisk indicate the residues that are likely to be phosphorylated by PKB/Akt. PKB/Akt phosphorylation sites 3, 4, 5, and 6 are conserved among mouse, rat, and human PDE3A sequences. (B) MA10 cells were transfected with full-length PDE3A-HA (WT-PDE3A) or amino-terminus truncated PDE3A-HA (ΔNcoI-PDE3A) (1st Tfn), and constitutively active PKB/Akt (myr-Akt) or kinase-dead PKB/Akt (KD-Akt) was transfected after 16 hours (2nd Tfn). Nineteen hours later, cilostamide-sensitive PDE activity was measured on total cell extracts. Only full length of PDE3A not ΔNcoI-PDE3A mutant is activated by myr-Akt transfection. Download figure Download PowerPoint To confirm whether these putative Akt phosphorylation sites are phosphorylated by myr-Akt, the following seven PDE3A mutants were generated: M1–6 mutant (S35A, S271A, S290A, S291A, S292A, S462A), M3 mutant (S290A), M4 mutant (S291A), M5 mutant (S292A), M6 mutant (S462A), M3–5 mutant (S290A, S291A, S292A), or M3–6 mutant (S290A, S291A, S292A, S462A) (Figure 3A). After serial transfection of myr-Akt and one of the PDE3A mutants, the immunoprecipitated complexes were blotted with phospho-Akt substrate antibodies. All of the immunoprecipitated mutants from intact cells expressing myr-Akt were phosphorylated except M1–6, which is a mutant of all of the putative sites (Figure 3B). Next, by measuring the PDE3A activity in the lysates of double-transfected cells, we defined whether PKB/Akt-dependent phosphorylation of PDE3A is important for activation. Only the WT and M6 mutant PDE3As were activated by myr-Akt (Figure 3C). Because all single mutated PDE3As in the M3, M4, and M5 sites were not activated by PKB/Akt (Figure 3C), we surmised that the three serines in the mouse PDE3A sequence, S290–292, are required for PKB/Akt-dependent activation, whereas the other putative phosphorylation sites, although perhaps physiologically relevant, do not impact on the PDE activity. This conclusion is further supported by the analysis of an additional construct mutated in S35, S271, and S462 to alanine (M1, 2, 6) but retaining the Akt putative sites (Supplementary Figure 3). After myr-Akt cotransfection, this mutant was phosphorylated to a lesser extent than the WT PDE3A, but was activated in a manner similar to WT PDE3A (Supplementary Figure 3). Figure 3.S290–292 serine residues in the PDE3A sequence are important for PKB/Akt-induced PDE3A activation. (A) M3, M4, M5, M6, M3–5, M3–6, M1, 2, 6, or M1–6 mutant PDE3A-HAs were generated as described in Materials and methods. The open circle indicates the site likely to be phosphorylated by PKB/Akt. The closed circle indicates the site that was mutated from serine to alanine. Amino-acid sequences of the S290–S292 region of PDE3A and mutant M3, M4, M5, and M3–5 are shown. Underlined alanine indicates the Ser/Ala mutation introduced. (B) The phosphorylation state of the various PDE3A mutants was determined using phospho-Akt substrate antibody after cotransfection with myr-Akt and immunoprecipitation. All of the immunoprecipitated mutants were phosphorylated except M1–6, which is a mutant of all of the putative sites. (C) Cilostamide-sensitive PDE activity was measured after serial transfection of Myr-Akt or empty vector (1st Tfn) and various PDE3A mutants (2nd Tfn). All single mutated PDE3As in the M3, M4, and M5 site were not activated by PKB/Akt and only the WT and M6 mutant PDE3As were activated by myr-Akt. ** represents P<0.01 compared to mock control. The expression levels of PDE3A and myr-Akt were confirmed with HA antibodies (lower panel; PDE3A, Myr-Akt). Download figure Download PowerPoint PDE3A phosphorylation mediates the insulin effects on Xenopus oocyte maturation To determine whether this PDE3A phosphorylation and activation by Akt is functionally significant in an intact cell model, we used oocyte maturation as a downstream readout for PDE activation. We have shown previously that PKB/Akt injection in Xenopus oocytes causes a two- to three-fold increase in PDE activity (Andersen et al, 1998). Increasing concentrations of mouse pde3a mRNA were injected into Xenopus oocytes and percentage of GVBD was measured. The induction of GVBD by overexpression of PDE3A was confirmed by activation (phosphorylation) of MAP kinase as reported (Figure 4A). Oocytes were then injected with 5 ng of WT pde3a mRNA followed by insulin treatment at a concentration that has a submaximal effect on GVBD (Andersen et al, 2003). Approximately 80–90% of the oocytes underwent GVBD (Figure 4B), and an approximate two-fold increase in PDE activation was measured in the oocyte extracts. Conversely, only 40% of GVBD was induced by insulin after injection of 10 ng of the M3–5 mutant form of pde3a mRNA (Figure 4B). When 10 ng mRNA was injected into the Xenopus oocytes, only the WT and M6 mutant induced more than 90% of GVBD with insulin, whereas less than 50% of GVBD was detected with the injection of the other mutants, a value comparable to water injection as a control (Figure 4C, middle panel). The induction of maturation was associated with an approximate two- to four-fold increase of PDE activity in Xenopus oocytes (Figure 4C, upper panel). Together with our previous report, these results strongly suggested that the PKB/Akt-stimulated PDE activity is required for insulin-dependent oocyte maturation in Xenopus oocytes. Figure 4.A two- to four-fold increase in PDE3A activity is sufficient to induce oocyte maturation. (A) Fifty nanograms of pde3a mRNA or H2O (vehicle) was injected into Xenopus oocytes. One group of H2O-injected oocytes was treated with 500 nM progesterone to induce oocyte maturation. Treatment of progesterone in the H2O-injected oocytes or PDE3A expression induces the phosphorylation of ERK (pERK), which is an established oocyte maturation marker. Protein expression (PDE3A) and the amount of loaded protein (ERK) were monitored by Western blot analysis. (B) Xenopus oocytes were injected with different amounts of pde3a mRNA (0.1–50 ng/oocyte) and treated with insulin. Twelve hours later, oocyte maturation (GVBD) was scored by the appearance of a white spot on the animal pole of oocytes. By injection with 5 ng WT pde3a mRNA (WT), more than 70% oocytes undergo GVBD, whereas only 40% of GVBD occurred by injection of 10 ng of the M3–5 mutant form (M3–5). The capacity of oocyte maturation is tested in H2O-injected oocyte by insulin or progesterone treatment (H2O+Ins, H2O+Prog). (C) Xenopus oocytes were injected with 10 ng of various mutants of PDE3A and treated with 1 μM of insulin. The maturation of the oocytes was scored and PDE3A activity was measured using the oocyte lysate. The WT and M6 mutant induced more than 90% of GVBD (middle panel) and this induction of maturation was associated with an approximate two- to four-fold increase of PDE activity (upper panel). Expression levels of the PDE3As were confirmed with HA antibodies (lower panel, PDE3A). Download figure Download PowerPoint Isoforms of PDE3A expressed in mouse oocyte It has been reported that at least three different immunoreactive forms of human PDE3As are present in cardiac myocytes (Movsesian, 2002). However, it is unclear whether these forms are generated by proteolysis, alternate splicing during transcription, or different methionine usage during translation. Previous report with oocytes had indicated the presence of multiple transcripts (Shitsukawa et al, 2001). Western blot analysis was performed with available PDE3A antibodies on extracts from WT and pde3−/− oocytes. As shown in Figure 5, an immunoreactive band of approximately 136 kDa is detected only in WT but not pde3−/− oocytes. The migration of this band corresponds to the size of full-length recombinant PDE3A. A more prominent smaller band of 118–120 kDa is expressed only in WT oocytes but not pde3−/− oocytes (Figure 5). Additional bands were considered nonspecific because they were still present in pde3a−/− oocytes. The same expression pattern of PDE3A was detected in WT and pde3−/− mice lung lysates (Figure 5), known to express the PDE3A. The experiments with two different antibodies showed comparable results (data not shown). On the basis of these findings, we surmised that the full-length PDE3A is expressed in mouse oocytes, together with a smaller form; however, the properties and the mechanism by which the lower form is generated are unclear. Figure 5.PDE3A expression in mouse oocytes. Increasing number of WT oocytes (lanes 3–7), WT (lane 8) or pde3−/− oocytes (lane 9, 112 oocytes/lane), and different amounts of lung lysate (lanes 12–15) were subjected to SDS–PAGE and protein detected with a PDE3A antibody (PDE3ACY). Different forms of PDE3A are expressed in the mouse oocyte. The double arrows indicate the 136 and 120 kDa specific bands. These two bands were not detected in oocyte (lane 9) and lung from pde3−/− animals (lanes 13, 15). Full-length recombinant PDE3A-transfected Hek293 cell lysate was used as size control (lanes 2, 11). Download figure Download PowerPoint Activation of Akt and PDE3A precedes activation of the MPF complex during in vivo maturation of mouse oocytes We investigated the time course of the activation of the Akt and maturation promoting factor (MPF) complexes (Cdc2/CyclinB) during in vivo mouse oocyte maturation. Mice were primed with PMSG for 44 h and then injected with hCG to induce oocyte maturation. Oocytes were collected and the activation of PKB/Akt was determined in extracts using Akt phospho-S473-specific antibodies. The phosphorylation of PKB/Akt reached a maximum 2 h after hCG injection and preceded MPF activation by 1 h (Figure 6A) and GVBD by 1.5–2 h. To determine whether PDE3A expressed in the oocyte is activated during the same time frame, extracts were immunoprecipitated with PDE3A antibody and activity measured in the immunoprecipitation pellet. The PDE3A activity recovered 2.5 h after hCG was significantly increased approximately by 30% (Figure 6B). Thus, activation of the PKB/Akt–PDE module does occur during oocytes maturation also in vivo. Figure 6.PKB/Akt and PDE3A activation precedes activation of the MPF complex. (A) Phosphorylation state of PKB/Akt at indicated hours after hCG injection was determined using Akt phosho-S473-specific antibodies (P-S473-Akt) and the amount of loaded protein was confirmed with Akt-specific antibodies (Tot Akt). Highly phosphorylated state of Akt is detected after 2 h from hCG injection. The value of p-Akt reports the ratio of phosphorylated Akt and total Akt (square). Activity of MPF complex (Cdc2/CyclinB) was measured with five randomly selected oocytes at each time point (triangle). The phosphorylation of histone H1, which is a substrate of MPF complex, started at 2 h after hCG injection and reached a maximum at 3 h (MPF). GVBD was counted by disappearance of the germinal vesicle or extrusion of the first polar body (filled circle). A representative of the three independent experiments performed is reported. *** represents P<0.005 compared to 0 h. (B) The mouse ovaries were removed and lysates immunoprecipitated with PDE3A antibody. The PDE3A activity was measured in the immune complex. After 2.5 h from hCG injection, 30% increase in PDE3A activity over basal was detected. The data are the mean±s.e.m. of three independent experiments. ** represents P<0.01 compared to 0 h. Download figure Download PowerPoint Expression of PDE3A rescues the phenotype of the pde3a−/− oocytes To investigate the impact of PDE3A phosphorylation during mammalian oocyte maturation, we injected WT and M3T mutant pde3a mRNA into oocytes from pde3a−/− mice. In oocytes where PDE3A is absent, PKA is maintained in an activated state by the elevated cAMP, thus preventing maturation in vitro and in vivo (Masciarelli et al, 2004). The injection of WT pde3a mRNA caused meiotic resumption in approximately 75% of oocytes, whereas only 30% of oocytes resumed meiosis when a similar concentration of the M3–5 mutant mRNA was used (Figure 7A, left panel). When M1, 2, 6 mutant was injected in the pde3−/− oocytes, 60% of oocytes underwent GVBD, at a rate similar to the WT PDE3A injection, but only 24% of M1–6 mutant resumed meiosis (Figure 7A, right panel). The expression of WT and mutant proteins was comparable (Figure 7A). Furthermore, injection of myr-Akt in mouse oocytes maintained in meiotic arrest with hypoxanthine caused meiotic resumption in approximately 80% of the oocytes and two-fold increase in PDE activity (Figure 7B). This effect is dependent on the expression of PDE3A because myr-Akt failed to induce meiotic maturation in pde3a null oocytes (Figure 7B, left panel). Furthermore, PDE3A activity is required for the Akt effect because inclusion of cilostamide prevents meiotic resumption (data not shown). Consistent with the observation in Xenopus oocytes, these findings confirm that PDE3A plays a role in mouse oocyte maturation, and that the putative PKB/Akt phosphorylation sites identified above (serines 290–292) are implicated in the regulation of PDE3A activity in these oocytes. Figure 7.PDE3A is required for Akt-induced mouse oocyte maturation. (A) Oocytes from pde3−/− mice were injected with mRNA of WT PDE3A, M3–5, M1,2,6, or M1–6 mutant form. Vehicle (H2O) was injected as a control. Maturation of mouse oocytes was monitored by disappearance of the germinal vesicle or extrusion of the first polar body 19 h after mRNA injection. The injection of WT or M1,2,6 PDE3A mRNA induced meiotic resumption around 60–75% of oocytes, whereas about 30% of oocytes resumed meiosis when M3–5 or M1–6 mRNA was used. Numbers above the bars indicate the number of GVBD stage oocytes out of total injected oocytes. Protein expression of the injected mRNAs was compared with HA antibodies using 44 oocytes per each lane (left panel) and using 39 oocytes with PDE3A antibody (right panel). (B) Oocytes from WT and pde3a−/− mice were collected in M2 media, and WT oocytes maintained in meiotic arrest with 3.5 mM hypoxanthine. Left panel: Oocytes were injected with myr-Akt mRNA or H2O as a control. Injection of myr-Akt causes approximately 80% of meiotic resumption in WT oocytes but not in pde3−/− mouse oocytes. Right panel: Total PDE activity was measured with injected oocytes as described in Materials and methods. Approximately, two-fold increase in PDE activity was detected in myr-Akt-injected oocytes in three independent experiments. *** represents P<0.005 and * represents P<0.05 compared to control. Download figure Download PowerPoint Discussion PKB/Akt activation is a critical intermediate step downstream of PI3-kinase in insulin and IGF-I signaling. Several PKB/Akt substrates have been identified including FKHR, GSK3, and BAD (Toker and Newton, 2000; Scheid and Woodgett, 2001; Downward, 2004), thus demonstrating the pleiotropic effects of this signaling cascade on cell functions such as metabolism, growth, and survival. In this study, we have identified the residues of PDE3A required for PKB/Akt-mediated phosphorylation. Ablation of these sites prevents Akt phosphorylation of the enzyme and blocks its activation in vitro and in intact cells. Moreover, PDE3A lacking these sites is functionally impaired in its ability to promote oocyte maturation. Because Akt promotes maturation in frog and mouse oocytes, and this effect requires PDE3A, w