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An inverse relationship between T cell receptor affinity and antigen dose during CD4 + T cell responses in vivo and in vitro

1999; National Academy of Sciences; Volume: 96; Issue: 17 Linguagem: Inglês

10.1073/pnas.96.17.9781

1091-6490

William A. Rees, Jeremy Bender, T. Kent Teague, Ross M. Kedl, Frances Crawford, Philippa Marrack, John W. Kappler,

Immunotherapy and Immune Responses

Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity to T cell hybridomas. A small, expanded population of T cells detected with one of these reagents in peptide-immunized C57BL/10 mice persisted for several months. This population expanded further on secondary immunization. Equating the extent of binding of this reagent to T cell receptor affinity, we saw little correlation of immunizing peptide dose to T cell receptor affinity at the peak of the primary response. However, there was an inverse relation between peptide dose and the apparent receptor affinity of the T cells that were present several months after a primary response or after a secondary stimulation either in vivo or in vitro .